Method for preventing and treating Pseudomonas solanacearum

Abstract

The invention discloses method for preventing and treating Pseudomonas solanacearum, comprising the following steps: culture and purification of ralstonia dolaanacearum: utilizing sterilized water to soak tobacco leaf vein with Pseudomonas solanacearum, screening on a culture medium, selecting single bacterial colonies with good fluidity, continuing to culture the single bacterial colonies with good fluidity, and storing purified ralstonia dolaanacearum; screening of bacteriophage: acquiring tobacca planting soil subjected to Pseudomonas solanacearum, sieving, removing impurities, acquiring fine particles, soaking the fine particles with sterilized water, centrifuging, acquiring a supernatant liquid, filtering, acquiring a filtered solution, culturing the filtered solution and a ralstonia dolaanacearum solution together, and storing screened bacteriophage; and enrichment culture and collection of the bacteriophage: adding the culture medium and sterilized water containing ralstonia dolaanacearum into a triangular flask, carrying out shake culture, then adding sterilized water containing bacteriophage, continuing to culture, centrifuging a culture solution, acquiring a supernatant liquid, and filtering to obtain a filtered solution for later use; soaking a toothpick with sterilized water, flushing, sterilizing, drying, cooling to room temperature, soaking in the filtered solution, taking out, sub-packaging, and storing for later use; and after tobacco is transplanted, inserting the toothpick to the stem base of a tobacco plant so as to prevent and treat the Pseudomonas solanacearum.

Description

technical field

[0001] The technology relates to the cultivation of tobacco, in particular to the prevention and treatment of tobacco bacterial wilt. Background technique

[0002] Tobacco bacterial wilt is a systemic disease caused by Ralstonia solanacearum infestation. It is a typical vascular disease. The most prominent symptom is wilting. Once the disease occurs, the whole plant often dies, causing significant economic losses to tobacco leaf production. In the production process of tobacco leaves, the control of tobacco bacterial wilt includes chemical agent control such as streptomycin, reasonable crop rotation such as tobacco and corn rotation, planting disease-resistant varieties, transplanting in advance, adjusting soil microorganisms and adjusting the transplanting period of tobacco seedlings, microbial Comprehensive measures such as pesticides such as Jinmiku and Kang Dilei (seeing Chinese invention patent: ZL 02151019.9) still cannot effectively prevent the occurre...