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Biological fungicide, as well as preparation method and application thereof

A biological inoculum and biological technology, applied in the field of biological inoculum and its preparation, can solve problems such as few reports, and achieve the effects of promoting growth and development, improving soil physical and chemical properties, and promoting growth

Active Publication Date: 2020-09-25
TOBACCO RES INST CHIN AGRI SCI ACAD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are few reports of acid-resistant biocontrol strains that are applied to bioremediation of acidic soil and have control effects on tobacco bacterial wilt and black shank

Method used

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  • Biological fungicide, as well as preparation method and application thereof
  • Biological fungicide, as well as preparation method and application thereof
  • Biological fungicide, as well as preparation method and application thereof

Examples

Experimental program
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Effect test

preparation example Construction

[0037] The present invention provides a method for preparing a biological inoculum. The Pseudomonas korea CLP-23 is inoculated into a liquid medium for liquid dark culture to obtain a fermentation broth; the fermentation broth is freeze-dried to prepare a dry powder to obtain Biological agents.

[0038] In the present invention, the Pseudomonas Koreana CLP-23 is inoculated into a liquid medium and cultured in the dark to obtain a biological agent. In the present invention, the inoculation preferably includes Pseudomonas korea CLP-23 activation treatment, and the activation preferably includes streaking Pseudomonas korea CLP-23 on a nutrient agar medium for activation and culture to obtain Korea A single colony of Pseudomonas CLP-23; the Pseudomonas Korea CLP-23 is preferably a Pseudomonas Korea CLP-23 strain frozen in 30% glycerol; the activation culture is preferably a dark culture, so The conditions for the activation culture are preferably: the temperature is preferably 28°C,...

Embodiment 1

[0049] CLP-23 antibacterial activity under different pH conditions

[0050] The preparation of NB medium with different pH is as follows: Titrate NB to pH 5.0, pH 5.5, pH 6.0, pH 6.5, pH 7.0, pH 7.5 and pH 8.0 with pH 4.0 and 8.0 acid-base buffers, sterilize at 121°C for 20 minutes, and reserve .

[0051] The antagonistic activity of strain CLP-23 against R. solanacearum was performed by agar punching method. The CLP-23 strain was inoculated into NB under the above pH conditions. The bacterial liquid after shaking culture at 28°C and 150r / min for 24h was used for the antibacterial activity determination. The bacterial liquid was centrifuged at 5000r / min for 5min under low temperature conditions, and the supernatant Obtain the sterile filtrate through a bacterial filter (pore size 0.22μm), use the NB culture solution as the blank CK, conduct a confrontation experiment on the NA plate, repeat each treatment 3 times, incubate at a constant temperature of 28°C for 48h, and measure the...

Embodiment 2

[0057] The destructive effect of CLP-23 on R.solanacearum

[0058] The preparation method of CLP-23 sterile filtrate: the fermentation broth of CLP-23 strain for 48 hours is centrifuged at 8000 rpm, 5 minutes under low temperature conditions, and then the bacterial pellet is discarded. .

[0059] The preparation method of Ralstonia solanacearum fermentation broth: inoculate CLP-23 bacteria into a liquid culture medium at 28° C., 120 rpm, and shake culture for 48 hours under dark conditions, namely fermentation broth.

[0060] Take 10 mL of CLP-23 sterile filtrate and 90 mL of Ralstonia solanacearum fermentation broth (10 8 cfu / mL). The blank control is treated with 10 mL of NB medium, with 3 replicates for each treatment. After mixing, the bacterial solution was centrifuged at low temperature at 5000r / min for 5min at the 24h and 48h after mixing. The bacterial pellet was collected and washed with sterile PBS buffer for 3 times, and then resuspended in 200μL PBS, and then fixed with ...

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Abstract

The invention belongs to the technical field of microbial prevention and control and particularly relates to a biological fungicide, as well as a preparation method and application thereof. The biological fungicide comprises pseudomonas koreensis CLP-23 which has a biological preservation number of CGMMCC No.13203. The biological fungicide can inhibit ralstonia solannacearum bacteria, can improvethe prevention and control effect on the ralstonia solannacearum under an acid soil condition, has a biologically soil repairing effect, and can promote growth of solanaceae.

Description

Technical field [0001] The invention belongs to the technical field of microbial control, and specifically relates to a biological fungus and a preparation method and application thereof. Background technique [0002] The gradual increase in the number of people and the rapid development of agriculture have caused the per capita arable land area to become more tense. In my country’s agricultural production, there is a widespread phenomenon of increasing crop cultivation years and expanding continuous cropping area. Various replanting obstacles have become prominent in production. problem. Mitigation of continuous cropping obstacles is usually achieved by adopting disease-resistant varieties selection and rotation cultivation system, but these methods have some limitations and shortcomings. The increase of continuous cropping years will still result in a substantial reduction in disease-resistant varieties; if the next crop is not selected properly , Rotation cultivation will brin...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/27A01P1/00A01P21/00C09K17/14A01G7/06C12R1/38
CPCC12N1/20A01N63/27C09K17/14A01G7/06
Inventor 王静王贻鸿冯永新王杰刘东阳雷霆
Owner TOBACCO RES INST CHIN AGRI SCI ACAD
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