Fermenting and wall-breaking process for rape pollen

A technology for rape pollen and a new method, which is applied in the application, food preparation, food science and other directions, can solve the problems of unsuitable promotion and implementation of industrialization, high requirements for equipment and working conditions, destruction of pollen nutrients, etc., and achieves low cost and production. Simple process, small damage effect

Inactive Publication Date: 2003-11-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] 1) The requirements for equipment and working conditions are high, and the investment is large, so it is not suitable for promotion and industrialization;
[0005] 2) The wall breaking rate is low;
[0006] 3) When using the temperature difference method, due to the excessive temperature change, the pollen nutrients are greatly destroyed

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1 pollen fermentation wall breaking method steps are as follows:

[0024] 1. Preparation of culture medium:

[0025] 1) Grind soybeans with a pulverizer, add water at a ratio of 1:10, cook in a pressure cooker for 65 minutes, filter with gauze, extract soybean juice, prepare a culture medium at a ratio of 10mg soybeans, 3mg sucrose, and 4mg agar, with a natural pH.

[0026] 2) Tiger Red (Bengal Red) medium: 5 g of peptone, 10 g of glucose, 1 g of potassium dihydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (0) 0.5 g, 20 g of agar, 100 ml of 1 / 3000 tiger bengal aqueous solution, 0.1 g of chloramphenicol, after adding 1000 ml of distilled water to dissolve, then adding the tiger bengal solution, subpackaging, and autoclaving (121° C., 20 min).

[0027] 3) Mold medium: 5g of peptone, 2g of yeast extract, 1g of dipotassium hydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (2) 0.5g, agar 20g, chloramphenicol 0.1g, add in 1000ml distilled water, dissolve a...

Embodiment 2

[0035] 1. Culture medium preparation:

[0036] 1) Grind the soybeans with a grinder, add water at a ratio of 1:12, wrap the soybeans in gauze, cook in a pressure cooker for 80 minutes, extract the soybean juice, prepare a medium according to the ratio of 12mg soybeans, 4mg sucrose, and 5mg agar, pH natural state.

[0037] 2) Tiger red (Bengal red) medium: 6 g of peptone, 12 g of glucose, 2 g of potassium dihydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (0) 0.6g, agar 22g, chloramphenicol 0.2g, add 1000ml of distilled water to dissolve, then add 120ml of 1 / 3000 tiger red aqueous solution, after subpackaging, autoclave (121°C, 30min).

[0038] 3) Mold medium: 6g of peptone, 3g of yeast extract, 1.5g of dipotassium hydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (2) 0.6g, agar 20g, chloramphenicol 0.2g, add in 1000ml distilled water, dissolve at tepid temperature, adjust pH to be 6.8, boil, add 20g glucose, after dissolving, filter and adjust pH to be 6.8, make pH af...

Embodiment 3

[0046] 1) Culture medium preparation:

[0047] (1) Soybeans are crushed with a pulverizer, water is added in a ratio of 1:8, soybeans are wrapped in gauze, steamed in a pressure cooker for 80 minutes, soybean juice is extracted, and a culture medium is prepared at a ratio of 8mg soybeans, 2mg sucrose, and 4mg agar, pH natural state;

[0048] (2) Tiger red (Bengal red) medium: 4 g of peptone, 8 g of glucose, 1 g of potassium dihydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (2) 0.3g, agar 18g, chloramphenicol 0.1g, add 1000ml distilled water after dissolving, then add 1 / 3000 tiger red aqueous solution 80ml, after packing, autoclave (121 ℃, 20min);

[0049] (3) Mold medium: 4g of peptone, 1g of yeast extract, 0.5g of dipotassium hydrogen phosphate, magnesium sulfate (MgSO 4 ·7H 2 (0) 0.6g, agar 18g, chloramphenicol 0.1g, add 1000ml distilled water, dissolve at low temperature, adjust pH to 6.5, boil, add 18g glucose, after dissolving, filter to adjust pH to 6.0, autoclav...

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PUM

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Abstract

The fermenting and wall-breaking process of rape pollen includes the following steps: preparing culture medium; disinfection and inoculation; preparing yeast; preparing enzyme solution through soaking the yeast in hot water, extruding and collecting the fermented liquid as the enzyme solution;enzyme solution fermentation; vacuum stoving rape pollen, crushing and sieving to obtain pollen particle;spraying anhydrous ethanol to sterilize and vacuum stoving; mixing pollen particle and enzyme solution; and culturing the mixture at 37 deg.c to obtain wall-breaking pollen. The said process has high wall-breaking rate, less damage to nutritive components, and improved pollen desensitization, debitterizing and deastruingent effects.

Description

technical field [0001] The invention relates to a novel method for breaking walls of rapeseed pollen fermentation. Background technique [0002] Pollen (pollen) is the male gametophyte of plants, the highly concentrated "micro-nutrition pool" of plants, which not only contains a variety of nutrients needed for human survival, but also contains a variety of biologically active substances, which have wonderful effects on various physiological functions of the body. Regulatory effects can enhance physical fitness, improve immunity, delay aging, lower blood pressure, etc., and are known as "complete nutrition". Pollen is composed of pollen wall and inclusions (or protoplasm, the main nutrients of pollen). The pollen wall can be divided into outer wall and inner wall. The outer wall is mainly composed of sporopollenin and cellulose, and the inner wall is mainly composed of cellulose and pectin. ; Pollen cell walls are difficult to break due to the uniqueness...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L21/20
Inventor 沈生荣于海宁白骅
Owner ZHEJIANG UNIV
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