Method for preparing beta-carotene

A carotene and organic solvent technology, applied in the field of preparation of β-carotene, can solve the problems of β-carotene oxidation loss, β-carotene oxidation, product quality is difficult to guarantee, etc., to reduce energy consumption and high wall breaking rate , The effect that is conducive to environmental protection

Inactive Publication Date: 2015-04-29
CABIO BIOTECH WUHAN CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the temperature and pressure used in this method are relatively high, which easily oxidizes β-carotene and affects the quality of the product.
Chinese patent application No. 201310003309.1 discloses a method of grinding and extracting dried mycelium of B. trispora. This method requires drying of the mycelium, which is likely to cause β-carotene oxidation loss
[0005] On the whole, the existing preparation of β-carotene crystals by microbial fermentation has the following disadvantages: complex process, high cost, and difficult to guarantee product quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Using the B. trispora species as the fermentation strain, follow the following steps successively:

[0017] 1) Inoculate and ferment B. trispora; 1 hour before the end of fermentation, add 0.3% helicase in the volume of the fermentation broth;

[0018] 2) The fermented liquid obtained after the fermentation is finished is obtained by plate and frame press filtration to obtain wet thallus;

[0019] 3) Take 300g of wet mycelia, add 3000ml of butane, and use a high-speed bead mill to circulate and shear and break until the average particle size of mycelia is 300um.

[0020] 4) Centrifuge the cell mixture to obtain the extract.

[0021] 5) Under the condition of 20°C, absorb the above extract with macroporous adsorption resin for 0.5h. Under the condition of 30° C., the macroporous adsorption resin was analyzed with ethyl acetate for 0.5 h to obtain an analysis solution containing β-carotene.

[0022] 6) After removing the solvent in the analytical solution, vacuum-dry t...

Embodiment 2

[0024] Using the B. trispora species as the fermentation strain, follow the following steps successively:

[0025] 1) Inoculate and ferment B. trispora; 4 hours before the end of fermentation, add alkaline protease with a volume of 0.15% of the fermentation broth;

[0026] 2) The fermented liquid obtained after the fermentation is finished is obtained by plate and frame press filtration to obtain wet thallus;

[0027] 3) Take 500g of wet mycelium, add 10000ml of ethyl acetate, and use a shearing machine to cut and break it until the average particle size of mycelium is 180um.

[0028] 4) Centrifuge the bacterial mixture to obtain the extract.

[0029] 5) Under the condition of 30°C, absorb the above extract with macroporous adsorption resin for 1.5h. Under the condition of 40° C., the macroporous adsorption resin was analyzed with butane for 1.5 h to obtain an analysis solution containing β-carotene.

[0030] 6) After removing the solvent in the analytical solution, vacuum-...

Embodiment 3

[0032] Using the B. trispora species as the fermentation strain, follow the following steps successively:

[0033] 1) Inoculate and ferment B. trispora; 24 hours before the end of fermentation, add chitinase with a volume of 0.1% of the fermentation broth;

[0034] 2) The fermented liquid obtained after the fermentation is finished is obtained by plate and frame press filtration to obtain wet thallus;

[0035] 3) Take 5kg of wet mycelium, add 75L of ethyl acetate, and use a shearing machine to cyclically shear and break the mycelium with an average particle size of 150um.

[0036] 4) Centrifuge the cell mixture to obtain the extract.

[0037] 5) Under the condition of 25°C, absorb the above extract with macroporous adsorption resin for 1 hour. Under the condition of 35° C., the macroporous adsorption resin was analyzed with petroleum ether for 1 h to obtain an analysis solution containing β-carotene.

[0038] 6) After removing the solvent in the analytical solution, vacuum-...

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PUM

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Abstract

The invention discloses a method for preparing beta-carotene. The method comprises the following steps: inoculating a microbial strain for fermentation; adding bio-enzyme for breaking walls 1 to 24 hours before the fermentation is ended; performing solid and liquid separation on a fermentation solution to obtain a wet cell; mixing the wet cell with an organic solvent, and circularly shearing and crushing; performing solid and liquid separation on the mixed solution to obtain an extraction solution; adsorbing the beta-carotene in the extraction solution by using macro-porous adsorption resin; desorbing the macro-porous adsorption resin by using the organic solvent after the adsorption is ended to obtain a desorption solution containing the beta-carotene; removing the solvent from the desorption solution and drying in vacuum to obtain a beta-carotene crystal. According to the method disclosed by the invention, the wet cell is high in wall breaking rate by combination of bio-enzyme wall breaking and mechanical wall breaking; therefore, the extraction rate of the beta-carotene is high; the strain needs not to be dried during extraction, so oxidation of the beta-carotene is avoided. On the other hand, in the method, the macro-porous adsorption resin is used for separating and purifying the beta-carotene, so the process is simple; the mixed oil generated after the beta-carotene is extracted can be recycled as an extraction solvent, so the cost can be lowered, energy consumption can be reduced and environmental protection can be facilitated.

Description

technical field [0001] The invention relates to a method for preparing beta-carotene, in particular to a method for preparing beta-carotene by fermenting microbial strains. Background technique [0002] Carotenoid is a kind of nutrient that widely exists in plants, animals, and microorganisms and is closely related to human health. It mainly includes β-carotene, lycopene, and lutein. Carotenoids not only have high medicinal value such as anti-cancer and anti-oxidation, but also are an important source of vitamin A for human body. As food additives and nutritional enhancers, carotenoids have been recognized by FDA, European Community, WHO and other international organizations. In recent years, they have been widely used in the fields of medicine, food, health products and cosmetics. [0003] Natural beta-carotene comes mainly from plants. However, plant-based raw materials have shortcomings such as insufficient supply and low content, and cannot be used on a large scale in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C403/24C12P23/00C12R1/89C12R1/645
Inventor 李翔宇田勇汪志明陆姝欢周强易德伟
Owner CABIO BIOTECH WUHAN CO LTD
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