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Separation and purification method of beta-glucan

A technology for separation and purification of glucan, applied in the field of separation and purification of β-glucan, to achieve the effect of reducing production operation costs, improving filtration effect, and not easily deformed

Inactive Publication Date: 2017-08-22
SICHUAN HUI TAI AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, there is no method for separating and purifying β-glucan without destroying the molecular structure and chemical quality of β-glucan

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] A method for separating and purifying β-glucan, comprising the following steps:

[0024] (1) Crush the rice, add water, stir and dissolve at 60°C, filter, and extract to obtain the initial solution containing β-glucan;

[0025] (2) Filter the initial solution with a sieve with a pore size of 0.5 mm, collect the filtrate, and obtain a separation liquid;

[0026] (3) Add an organic solution mixed with isopropanol and petroleum ether at a volume ratio of 1:5 to the separation liquid, degrease for 30 minutes, then add 85% ethanol, adjust the pH of the separation liquid to 8.5 with ammonia water, and inactivate the enzyme at 85°C 4.5h; wherein, the volume ratio of the organic solution to the separation liquid is 1:12, and the volume ratio of ethanol to the separation liquid is 1:15;

[0027] (4) Using 3D printing technology, using nickel-based alloy powder as raw material, a metal filter membrane with a pore size of 0.3 μm was prepared, and the solution obtained in the step...

Embodiment 2

[0030] A method for separating and purifying β-glucan, comprising the following steps:

[0031] (1) Crush tomatoes, add water, stir and dissolve at 90°C, filter, and extract to obtain an initial solution containing β-glucan;

[0032] (2) Filter the initial solution with a sieve with a pore size of 0.5 mm, collect the filtrate, and obtain a separation liquid;

[0033] (3) Add an organic solution mixed with isopropanol and petroleum ether at a volume ratio of 1:5 to the separation liquid, degrease for 30 minutes, then add 85% ethanol, adjust the pH of the separation liquid to 8.5 with ammonia water, and inactivate the enzyme at 85°C 4.5h; wherein, the volume ratio of the organic solution to the separation liquid is 1:12, and the volume ratio of ethanol to the separation liquid is 1:15;

[0034] (4) Using 3D printing technology, using nickel-based alloy powder as raw material, a metal filter membrane with a pore size of 0.3 μm was prepared, and the solution obtained in the step ...

Embodiment 3

[0037] A method for separating and purifying β-glucan, comprising the following steps:

[0038] (1) Crush apples, add water, stir and dissolve at 84°C, filter, and extract to obtain an initial solution containing β-glucan;

[0039] (2) Filter the initial solution with a sieve with a pore size of 0.5 mm, collect the filtrate, and obtain a separation liquid;

[0040] (3) Add an organic solution mixed with isopropanol and petroleum ether at a volume ratio of 1:5 to the separation liquid, degrease for 30 minutes, then add 85% ethanol, adjust the pH of the separation liquid to 8.5 with ammonia water, and inactivate the enzyme at 85°C 4.5h; wherein, the volume ratio of the organic solution to the separation liquid is 1:12, and the volume ratio of ethanol to the separation liquid is 1:15;

[0041] (4) Using 3D printing technology, using nickel-based alloy powder as raw material, a metal filter membrane with a pore size of 0.3 μm was prepared, and the solution obtained in the step (3...

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PUM

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Abstract

The invention discloses a separation and purification method of beta-glucan, and the method comprises the following steps: (1) extraction of a beta-glucan solution; (2) filtration; (3) defatting and enzyme deactivation; (4) refiltration; and (5) pressure filtration. When the method is used for separation and purification of the beta-glucan, the molecular structure and chemical quality of the beta-glucan cannot be destroyed, the purity and yield of the beta-glucan can be effectively improved, and the whole separation process is pollution-free, green, environment-friendly and easy to realize industrial popularization.

Description

technical field [0001] The invention belongs to the technical field of separation and purification, and in particular relates to a separation and purification method of β-glucan. Background technique [0002] Studies in recent years have shown that β-glucan has the main functions of lowering cholesterol, lowering blood fat, lowering blood sugar and improving human immunity, and it is processed into health products. At present, there have been studies on the extraction of β-glucan from cereals, barley and other crops. [0003] At present, the methods for extracting β-glucan mainly include hot water method, acid method, alkali method and ultrasonic method. When considering which extraction method to choose, factors such as extraction rate and purity of β-glucan, avoidance of enzymatic hydrolysis of β-glucan, production cost, and ease of industrialization need to be considered. [0004] At present, there is no method for separating and purifying β-glucan without destroying th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/02
CPCC08B37/0024
Inventor 陈乐章杨军波谢尚明鲍向义
Owner SICHUAN HUI TAI AGRI TECH CO LTD
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