Sterile cultivation method of mealworms

A technology of Tenebrio molitor and bran culture medium, which is applied in animal husbandry and other directions, can solve the problems of Tenebrio molitor's death, interfere with the accuracy of experiments, etc., and achieve the effect of simple operation and good effect.

Inactive Publication Date: 2013-05-15
BAOSHAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In the practice of using Tenebrio molitor to trap soil pathogenic fungi, Tenebrio molitor itself carries the

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] (1) Treatment of the first-generation Tenebrio molitor material: select healthy Tenebrio molitor larvae, wash the surface for 3 times with sterile water, immediately dry the water on the worm body with sterile filter paper, and disinfect in 75% disinfectant alcohol for 5 Seconds, evaporate the alcohol on a sterile filter paper sheet, and use the processed larva as the parent material.

[0016] (2) Preparation of sterile bran medium: take 30g bran and put it in a 500ml can bottle, seal the bottle mouth, sterilize at 0.11MPa, 121°C for 60min, and cool for later use.

[0017] (3) Aseptic culture of Tenebrio molitor: the prepared first-generation Tenebrio molitor was placed in canned bottles of sterile bran medium in the amount of 10 per bottle, and cultured at 15° C. until the larvae pupated. Aseptically transfer the pupae to a fresh aseptic bran medium, and then transfer them to a fresh aseptic bran medium after pupae eclosion, and transfer the mated adults to a sterile w...

Embodiment 2

[0020] (1) Treatment of the first-generation Tenebrio molitor material: select healthy Tenebrio molitor larvae, wash the surface three times with sterile water, immediately absorb the water on the worm body with sterile filter paper, and sterilize in 75% disinfectant alcohol for 10 days. Seconds, evaporate the alcohol on a sterile filter paper sheet, and use the processed larva as the parent material.

[0021] (2) Preparation of sterile bran medium: take 30g bran and put it in a 500ml can bottle, seal the bottle mouth, sterilize at 0.11MPa, 121°C for 60min, and cool for later use.

[0022] (3) Aseptic culture of Tenebrio molitor: the prepared first-generation Tenebrio molitor was placed in canned bottles of sterile bran medium in the amount of 10 per bottle, and cultured at 20° C. until the larvae pupated. Aseptically transfer the pupae to a fresh aseptic bran medium, and then transfer them to a fresh aseptic bran medium after pupae eclosion, and transfer the mated adults to a...

Embodiment 3

[0025] (1) Treatment of the first-generation Tenebrio molitor materials: select healthy Tenebrio molitor larvae, wash the surface 3 times with sterile water, immediately absorb the water on the worm body with sterile filter paper, and disinfect in 75% disinfectant alcohol for 15 Seconds, evaporate the alcohol on the sterile filter paper, and the treated larvae are used as the primary material.

[0026] (2) Preparation of sterile bran medium: take 30g bran and put it in a 500ml can bottle, seal the bottle mouth, sterilize at 0.11MPa, 121°C for 60min, and cool for later use.

[0027] (3) Aseptic culture of Tenebrio molitor: the prepared first-generation Tenebrio molitor was placed in canned bottles of sterile bran medium in the amount of 10 per bottle, and cultured at 25° C. until the larvae pupated. Aseptically transfer the pupae to a fresh aseptic bran medium, and then transfer them to a fresh aseptic bran medium after pupae eclosion, and transfer the mated adults to a sterile...

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PUM

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Abstract

The invention relates to a sterile cultivation method of mealworms. The operation steps mainly comprise processing of early generation mealworm materials, preparation of sterile bran culture media, sterile cultivation of the mealworms, and detection of sterile imagoes and quantity statistics. Through using of the provided sterile cultivation method of the mealworms, purposes that imago bodies do not carry germs, and the mealworms grow healthily and strongly are achieved. The sterile cultivation method of the mealworms is easy and convenient to operate, good in effects, and capable of being applied to material drawing experiments of sterile materials each stage of the life history of the mealworms.

Description

technical field [0001] The invention belongs to the field of biological technology, and relates to a method for aseptically cultivating Tenebrio molitor. Background technique [0002] Tenebrio molitor (Tenebrio molitor L.) belongs to the Coleoptera, Pseudomidae, Pseudocaria family and Pseudocaria genus in classification. It is a large storage pest and a resource insect with great development potential. The insects are mainly used as feed for poultry and some other special economic animals (scorpions, geckos, bullfrogs, forest frogs, turtles, scorpions, snakes, centipedes, ornamental fish and birds, etc.), and are also used to develop health food or medicine (Bai Yaoyu , Cheng Jiaan, Insect Knowledge, 2003, 40(4): 317-322). [0003] Tenebrio molitor has miscellaneous feeding habits, wide food sources, strong vitality, and is easy to raise artificially. It is an ideal material for trapping insects of pathogenic fungi. Jia Chunsheng et al. used Tenebrio molitor to isolate so...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 徐玲陈自宏
Owner BAOSHAN UNIV
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