Selective breeding technique of anthocyanin type purple corn
A purple corn and anthocyanin technology, applied in the direction of angiosperms/flowering plants, plant genetic improvement, botanical equipment and methods, etc., can solve the problems of inconvenient picking, storage, transportation, strict cultivation technology requirements, and low raw material output , to achieve the effects of simple cultivation techniques, high yield per unit area, and high anthocyanin content
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Embodiment 1
[0029] 1. Breeding of Shennong ZS717 purple corn inbred line
[0030] The foreign purple corn material Stock 6 with purple plants, bracts, grains and cobs was used as the female parent, and the Chinese white waxy corn Zhongnuo 1 was used as the male parent to breed ZS717 purple corn through the two-ring breeding method. Corn inbred lines:
[0031] F1 selects a single plant with purple plants, bracts, peels, and cobs for selfing. After harvest, the endosperm is selected by the minimally invasive single-seed identification method, and waxy, white single-seeds are selected;
[0032] F2~F3, select according to the method of F1;
[0033] F4, select the single plant selfing of plant, bract leaf, pericarp, and cob purple, and obtain endosperm waxy quality, endosperm white homozygous ear after harvesting;
[0034] F5, select the homozygous plants, bracts, pericarp, axis color, and Bainuo homozygous ears for selfing, and obtain excellent single plants;
[0035] F6, carrying out str...
Embodiment 2
[0041] 1. Breeding of Shennong ZF740 purple corn inbred line
[0042] Based on the population materials containing purple plants, purple husk leaves, purple peel, purple cobs, purple filaments and waxy traits, purple maize inbred lines were bred through one-ring breeding method:
[0043] S1, select a single plant with purple purple plants, bracts, peels, and cobs for selfing; after harvesting, identify the grains with a minimally traumatic single-seed identification method to obtain a single-seed homozygous for waxy endosperm;
[0044] S2, select a single plant with purple purple cobs, bracts, peels, and cobs for selfing; identify the grains of the harvested single ears with a micro-trauma single-seed identification method, and obtain single ears and single grains that are homozygous for waxy quality and white endosperm;
[0045] S3, select the single plant selfing of plant, bract leaf, pericarp, and cob purple; identify the grain of the harvested ear with the method of micro-...
Embodiment 3
[0055] 1. Breeding of Shennong ZS513 purple corn inbred line
[0056] The foreign purple corn material Stock 6 with purple plants, bracts, grains and cobs was used as the female parent, and the Chinese white waxy corn Zhongnuo 1 was used as the male parent to breed ZS717 purple corn through the two-ring breeding method. Corn inbred lines:
[0057] F1 selects a single plant with purple plants, bracts, peels, and cobs for selfing. After harvesting, use the micro-trauma identification method to identify single grains, and select single grains with white endosperm and non-waxy quality;
[0058] F2~F3, select according to the method of F1;
[0059] F4, select a single plant with purple purple cobs, bracts, peels, and cobs for selfing, and obtain a homozygous ear with white endosperm and non-waxy quality after harvest;
[0060] F5, obtain homozygous panicle rows with homozygous plant, bract leaf, pericarp, axis color, and white endosperm, and select excellent single plants for se...
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