Preparation method of exosome and stem cell proliferation reagent containing exosome

Abstract

The invention discloses a preparation method of exosome and a stem cell proliferation reagent containing the exosome. The preparation method of the exosome comprises the steps that 1, a culture mediumwithout exosome serum is added bone marrow mesenchymal stem cells to dilute the bone marrow mesenchymal stem cells into a cell suspension, the cell suspension is inoculated into a cell culture bottleto be cultured, supernate obtained after culture is collected, and the exosome is extracted; 2, cell fragments, dead cells and impurities in the supernate are removed; the supernate is removed, thena phosphate buffer salt solution (PBS) is added to dissolve and collect exosome precipitate, the exosome precipitate is filtered through a sterile filter membrane, and the obtained exosome precipitateis packaged in a sterile centrifugal tube and stored for later use. According to the method, in-vitro experiments prove that the proliferation activity and related functions of the mesenchymal stem cells can be remarkably promoted by co-culturing the exosome secreted by the early-generation bone marrow mesenchymal stem cells and human umbilical cord mesenchymal stem cells, and the method is an effective strategy for potentially improving the function of cells in a large-scale cell culture process.

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