Michelia compressa tissue culture propagation method

A technology of tissue culture and culture medium, applied in the field of plant biology, can solve the problems of staying in place, failure of rooting treatment, and far difference in tissue culture effect, etc., to achieve the effect of promoting fullness of axillary buds, obvious increase in height, and rapid growth
CN103392601AActive Publication Date: 2013-11-20NANJING JINPU LANDSCAPE
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING JINPU LANDSCAPE
Publication Date
2013-11-20

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Abstract

The invention discloses a michelia compressa tissue culture propagation method. The method includes the following steps: culturing explant plants; selecting the explants and disinfecting; culturing by an improved WPW culture medium; after cutting the treated explants, carrying out axillary bud early-generation induction culture; carrying out high voltage electrostatic field treatment; carrying out transgenerational multiplication culture; carrying out rooting induction culture; and thus successfully obtaining root-containing tissue culture seedlings by the tissue culture propagation method of the invention, wherein the proliferation multiple is 86 times, the rooting rate is 82%, the average rooting number is 3.1, and the exercising seedling survival rate reaches 90%. The method can efficiently obtain the michelia compressa seedlings, and greatly meets the demand of markets.
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Description

technical field

[0001] The invention belongs to the field of plant biotechnology, in particular to a propagation method of Taiwan Michelia compressa tissue culture. Background technique

[0002] Michelia compressa from Taiwan belongs to the plant of the genus Michelia L. in the family Magnoliaceae. Large evergreen tree, bark taupe, slightly smooth. The leaves are entire or wavy, leathery, and the leaf buds and flower buds are covered with reddish brown hairs. Among the evergreen broad-leaved tree species, the landscape effect of Taiwan Michelia is outstanding, the tree shape is compact, the trunk is strong, the crown is nearly conical, the side branches are all obliquely extending upwards and approaching the main trunk, and the dark green and shiny leaves are densely grown on 1, The tops of the 2-year-old branches form a green and dense crown in all seasons. The flowers are petite and elegant, and the breeze blows gently, exuding a faint fragrance. Through propagation an...

Claims

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