Michelia compressa tissue culture propagation method

Abstract

The invention discloses a michelia compressa tissue culture propagation method. The method includes the following steps: culturing explant plants; selecting the explants and disinfecting; culturing by an improved WPW culture medium; after cutting the treated explants, carrying out axillary bud early-generation induction culture; carrying out high voltage electrostatic field treatment; carrying out transgenerational multiplication culture; carrying out rooting induction culture; and thus successfully obtaining root-containing tissue culture seedlings by the tissue culture propagation method of the invention, wherein the proliferation multiple is 86 times, the rooting rate is 82%, the average rooting number is 3.1, and the exercising seedling survival rate reaches 90%. The method can efficiently obtain the michelia compressa seedlings, and greatly meets the demand of markets.

Description

technical field [0001] The invention belongs to the field of plant biotechnology, in particular to a propagation method of Taiwan Michelia compressa tissue culture. Background technique [0002] Michelia compressa from Taiwan belongs to the plant of the genus Michelia L. in the family Magnoliaceae. Large evergreen tree, bark taupe, slightly smooth. The leaves are entire or wavy, leathery, and the leaf buds and flower buds are covered with reddish brown hairs. Among the evergreen broad-leaved tree species, the landscape effect of Taiwan Michelia is outstanding, the tree shape is compact, the trunk is strong, the crown is nearly conical, the side branches are all obliquely extending upwards and approaching the main trunk, and the dark green and shiny leaves are densely grown on 1, The tops of the 2-year-old branches form a green and dense crown in all seasons. The flowers are petite and elegant, and the breeze blows gently, exuding a faint fragrance. Through propagation an...

AI Technical Summary

Problems solved by technology

Zeng Songjun and Peng Xiaoming used the dormant buds and the epicotyls and hypocotyls of the seedlings when the seeds germinated to induce the callus and adventitious buds of Shenshanxiao (Tissue Culture and Rapid Propagation of Shenshanxiao, Tropical and Subtropical Acta Botanica, No. 3, 2000, pp. 264-268); Chen Yunxia's paper conducted a preliminary study on Taiwan Michelia rapid propagation technology, however, it only obtained a multiplication coefficient of 2.906, which was at a very low level. The effects are far from each other, and all the rooting treatments studied have not been able to take root, and have not successfully induced rooting. Therefore, there is no successful tissue culture propagation with rooted seedlings.

[0004] At present, the tissue culture and propagation method of Michelia taiwanensis still stays at a relatively primary stage. The present invention provides a method for the tissue culture of Michelia taiwanensis tissue culture, which is simple and quick to operate, greatly improves the multiplication rate and multiplication speed, and obtains root-containing aseptic seedlings. Thus, it can meet the needs of Taiwan Michelle in urban landscaping, water conservation, soil and water conservation, green landscape passages, ecological construction, etc.

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