Cultivation method of maize inbred line

Abstract

The invention discloses a cultivation method of a maize inbred line, comprising the following steps of: (1) selective breeding of an induced line ''Lanyu induced No.1'' with high induction frequency from inductive materials of different sources; (2) induction method: taking the inductive materials as female parent and the ''Lanyu induced No.1'' as male parent according to the breeding objective to perform a test-cross, firstly selecting a female parent plant with normal growth to remove tassel, holding up pistil before wiredrawing, pollinating female parent by using pollen of ''Lanyu induced No.1'' when wiredrawing of filament finishes, quickly carrying out pollination and simultaneously taking off paper bags, holding up the paper bags after pollination, hanging with tags and fixing, performing ten panicles for each test-cross combination, harvesting and drying by sun according to test-cross combination; (3) selecting of monoploid grain; (4) identifying and treating of monoploid seedling; (5) enhancing field fertilizer and water management as well as cultivating and weeding; (6) early generation test-cross: inducing the duplex second generation inbred line into normal growth and development, recovering to normal growth of plant morphological characteristics, screening the inbred line by early generation test-cross to obtain a qualified maize inbred line. The invention can be used to cultivate a rapid and stable maize inbred line.

Description

technical field

[0001] The invention relates to a crop breeding method, in particular to a method for cultivating a corn inbred line. Background technique

[0002] At present, with the rapid development of the economy, the demand for corn in industry, animal husbandry, and biological industries is growing rapidly, which requires corn research to cultivate more high-yield, good-quality, disease-resistant, wide-adaptable, Different types of new varieties of corn with strong reversibility. Good new maize varieties are hybridized from excellent inbred lines with high yield and good combining ability. Therefore, breeding excellent inbred lines is the most important task of maize breeding. Conventional corn inbred breeding methods are generally "comprehensive selection method" and "two-ring selection method". In recent years, "anther (powder) cultivation method" has emerged. The latter is difficult to carry out due to its complicated technology, expensive medicines, many experim...