Cultivation method of maize inbred line

A cultivation method and technology of inbred lines, applied in the field of crop breeding, can solve the problems of difficult development, long time, complicated technical difficulty, etc., and achieve the effects of strengthening field fertilizer and water management, strengthening cultivating and weeding, and improving selection effect.

Inactive Publication Date: 2012-02-01
沈天民
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AI Technical Summary

Problems solved by technology

Conventional corn inbred breeding methods are generally "comprehensive selection method" and "two-ring selection method". In recent years, "anther (powder) cultivation method" has emerged. The latter is technically difficult, expensive drugs, many experimental instruments and equipment, and large investment, so it is difficult to carry out
The induction frequency of haploid breeding carried out internationally is low, which is more difficult, and it is difficult to be widely used in breeding

Method used

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Embodiment Construction

[0021] The present invention will be described in detail below in conjunction with specific embodiments, where the exemplary embodiments and descriptions of the present invention are used to explain the present invention, but are not intended to limit the present invention.

[0022] Follow the steps below to breed maize inbred lines:

[0023] (1) Material selection adopts the method of mutual hybridization and repeated screening, and selects the induction line "Lanyuyu No. 1" with high induction frequency from induction materials from different sources;

[0024] (2) Induction method: According to the breeding goal, the induction material is used as the female parent, and "Lanyuyu No. 1" is used as the male parent for testing and mating. Firstly, the normal growth plants of the female parent are selected to pull out the tassels, and the female ears are wrapped in a parchment paper bag before silking. Or the sulfate bag is tightly locked to avoid receiving external pollen. After...

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Abstract

The invention discloses a cultivation method of a maize inbred line, comprising the following steps of: (1) selective breeding of an induced line ''Lanyu induced No.1'' with high induction frequency from inductive materials of different sources; (2) induction method: taking the inductive materials as female parent and the ''Lanyu induced No.1'' as male parent according to the breeding objective to perform a test-cross, firstly selecting a female parent plant with normal growth to remove tassel, holding up pistil before wiredrawing, pollinating female parent by using pollen of ''Lanyu induced No.1'' when wiredrawing of filament finishes, quickly carrying out pollination and simultaneously taking off paper bags, holding up the paper bags after pollination, hanging with tags and fixing, performing ten panicles for each test-cross combination, harvesting and drying by sun according to test-cross combination; (3) selecting of monoploid grain; (4) identifying and treating of monoploid seedling; (5) enhancing field fertilizer and water management as well as cultivating and weeding; (6) early generation test-cross: inducing the duplex second generation inbred line into normal growth and development, recovering to normal growth of plant morphological characteristics, screening the inbred line by early generation test-cross to obtain a qualified maize inbred line. The invention can be used to cultivate a rapid and stable maize inbred line.

Description

technical field [0001] The invention relates to a crop breeding method, in particular to a method for cultivating a corn inbred line. Background technique [0002] At present, with the rapid development of the economy, the demand for corn in industry, animal husbandry, and biological industries is growing rapidly, which requires corn research to cultivate more high-yield, good-quality, disease-resistant, wide-adaptable, Different types of new varieties of corn with strong reversibility. Good new maize varieties are hybridized from excellent inbred lines with high yield and good combining ability. Therefore, breeding excellent inbred lines is the most important task of maize breeding. Conventional corn inbred breeding methods are generally "comprehensive selection method" and "two-ring selection method". In recent years, "anther (powder) cultivation method" has emerged. The latter is difficult to carry out due to its complicated technology, expensive medicines, many experim...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/08
Inventor 沈天民
Owner 沈天民
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