Improved protocol for random mutagenesis

The use of a free radical scavenger in conjunction with mutagens like EMS or ENU in plant cells addresses the issue of seed germination inhibition and mutation frequency compromise, enhancing plant development and yield in mutagenesis protocols.

WO2026115077A1PCT designated stage Publication Date: 2026-06-04KEYGENE NV
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Patent Information

Authority / Receiving Office
WO · WO
Patent Type
Applications
Current Assignee / Owner
KEYGENE NV
Filing Date
2025-11-28
Publication Date
2026-06-04

AI Technical Summary

Technical Problem

Existing chemical mutagenesis protocols for plant cells result in high inhibition of seed germination, compromising the germination quality and mutation frequency, necessitating an improved protocol that maintains high mutation frequency while minimizing detrimental effects on plant development.

Method used

A method involving exposure of plant cells to a mutagen, such as alkylating agents like EMS or ENU, in conjunction with a free radical scavenger like resveratrol, to reduce mutagen-induced detrimental effects on plant development and enhance germination and mutation frequency.

Benefits of technology

The method enhances plant survival rates, germination rates, and mutation frequency, resulting in a higher yield of mature and seed-bearing mutagenized plants, while maintaining similar mutation frequencies compared to protocols without scavengers.

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Abstract

The current invention pertains to a use of a scavenger in method or protocol for treatment of a plant cell with a mutagen, optionally in a method for random mutagenesis, mutagenized plants obtained using said method or protocol, and a composition or kit for use in said method or protocol.
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Citation Information

Patent Citations

  • Strategies for high throughput identification and detection of polymorphisms

    WO2006137733A1

  • High throughput screening of mutagenized populations

    WO2007037678A2

  • Method for high-throughput AFLP-based polymorphism detection

    WO2007073165A1

  • High throughput detection of molecular markers based on restriction fragments

    WO2007114693A2