A method for inducing hybrid orchid polyploid plants using compound mutagen

A compound mutagenesis and polyploidy technology, applied in the fields of botanical equipment and methods, applications, plant genetic improvement, etc., can solve the problems of high toxicity and large dosage of mutagen, achieve polyploid stability, and improve the mutation rate. , the effect of using a small dose

Active Publication Date: 2017-12-26
FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to solve the problems of high toxicity and large dosage of existing mutagens, the present invention provides a method for inducing polyploid plants of hybrid orchids by using compound mutagens, so as to increase the mutagenesis rate of hybrid orchids, reduce costs, and shorten identification time

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026](1) Preparation of chemical mutagen: Weigh 0.01g of asulphin and dissolve it in 1g of dimethyl sulfoxide (DMSO), then add distilled water to 100mL to obtain the mother liquor, and then dilute the mother liquor with distilled water to a concentration of 0.001~ 0.003%, and then sterilized at 121°C for 20 minutes, and after cooling, an asulphin solution with a mass concentration of 0.001-0.003% was obtained;

[0027] (2) Selection of processing materials: Hybrid orchids are induced to form rhizomes according to the conventional shoot tips and cultivated for one month. Then, select the young rhizome tips with the same size and location, cut off 1 cm, and soak them in the placenta obtained in step (1). Soak in sulfonyl solution for 24 hours, then wash with sterilized distilled water for 3 times, dry the water with sterilized filter paper, and then inoculate into the following medium: MS+EMS20mg / L+6-BA2.0mg / L+ NAA0.5mg / L+peptone 2g / L+sucrose 25g / L+banana 100g / L+activated carbo...

Embodiment 2

[0036] (1) Preparation of chemical mutagen: Weigh 0.01g of asulphin and dissolve it in 1g of dimethyl sulfoxide (DMSO), then add distilled water to 100mL to obtain the mother liquor, and then dilute the mother liquor with distilled water to a concentration of 0.001~ 0.003%, and then sterilized at 121°C for 20 minutes, and after cooling, an asulphin solution with a mass concentration of 0.001-0.003% was obtained;

[0037] (2) Selection of processing materials: Hybrid orchids are induced to form rhizomes according to the conventional shoot tips and cultivated for one month. Then, select the young rhizome tips with the same size and location, cut off 1 cm, and soak them in the placenta obtained in step (1). Soak in sulfonyl solution for 72 hours, then wash with sterilized distilled water for 3 times, blot dry with sterilized filter paper, and then inoculate into the following medium: MS+EMS70mg / L+6-BA2.0mg / L+ NAA0.5mg / L+peptone 2g / L+sucrose 25g / L+banana 100g / L+activated carbon 3g...

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Abstract

The invention discloses a method for inducing polyploid plants of hybrid orchids by using a compound mutagen. The preparation of the chemical mutagen, the selection and treatment of materials, the differentiation and cultivation of rhizomes, the morphological identification of mutant plants, the identification of stomata, and the identification of chromosomes , subculture, and finally determined to be hybrid orchid polyploid plants. The invention improves the mutagenesis rate of the hybrid orchid, the polyploid mutagenesis rate is increased by 10-20% on average, the highest death rate is 10%, and the chimerism rate is reduced by 10%. The invention has the advantages of reducing the formation of chimeras, increasing the mutation rate of polyploids, stabilizing polyploids after three generations, and shortening the identification time. The invention can increase the mutation rate of polyploid, reduce the damage to plants, and improve the survival rate of mutant seedlings. The dosage of Ansulphin and EMS is small, which can reduce the poison of chemical drugs and reduce the cost. And can effectively reduce the formation of chimeras and reduce costs.

Description

technical field [0001] The invention relates to a method for inducing hybrid orchid polyploid plants by using a compound mutagen, and belongs to the technical field of plant polyploid induction breeding. Background technique [0002] Hybrid orchid is a new variety bred by crossing Cymbidium and Cymbidium. At present, there are more than a dozen hybrid orchid varieties in China, such as Dafeng, Golden Prodigy, Purple Element, Ballet and so on. The hybrid orchid has the characteristics of large flowers and bright colors of the Cymbidium and the elegant fragrance of the Chinese orchid, and is deeply loved by people at home and abroad. In recent years, orchid breeders in the United States, Japan, New Zealand and other countries have crossed Cymbidium with Jianlan and Pleated Orchid, and have bred some early-flowering, slightly fragrant, short-leaf or pendant-flowering Cymbidium varieties. popular with consumers. Hybrid orchids make up for the lack of fragrance of Cymbidium cym...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 李涵曹桦闻永慧杨维李慧敏苗振黎霞赵培飞
Owner FLOWER RES INST OF YUNNAN ACAD OF AGRI SCI
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