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Method for induction mutating woody plant by using ethylmethane sulfonate

A technology of ethyl methanesulfonate and woody plants, which is applied in botany equipment and methods, plant cells, plant regeneration, etc., can solve the problems of long growth cycle, large individual, low mutation rate, etc., and achieve less drugs , small size, and the effect of increasing the mutagenesis rate

Inactive Publication Date: 2009-04-15
上海光兆植物速生技术有限公司
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Problems solved by technology

For woody plants, such as poplar, if these methods are also used to obtain variants, many difficulties will be encountered in actual operation, because they are large in size, have a long growth cycle, and are limited by the low mutation rate, so they must be processed. A large number of individuals can obtain the target number of variants, which is difficult to achieve in practice except for embryogenic cells

Method used

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Embodiment Construction

[0024] A method for mutagenizing woody plants using EMS, using woody plant callus embryogenic cells as mutagenic objects, including selection and treatment of explants, dedifferentiation culture, redifferentiation culture, mutagenesis treatment and screening, Wherein, the explants are tissue-cultured seedlings of woody plants, and the stem segments cut into 1-2 mm are inoculated in the culture medium, and after dedifferentiation culture and redifferentiation culture, EMS is used for mutagenesis treatment.

[0025] The mutagenesis treatment method comprises immersing the embryogenic cells of the woody plant callus in the EMS treatment solution for cultivation.

[0026] Adopt the method for EMS mutagenesis woody plant, select poplar callus embryogenic cell as mutagenesis object, comprise the following steps:

[0027] Step 1: Populus × euramericanaclGuangzhao No1. After multiple generations of tissue culture, select the tissue culture seedlings of excellent lines as explants;

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Abstract

The invention relates to a method for using methanesulfonic acid carbethoxy to induce woody plant, especially using callus blast cell to induce the methanesulfonic acid carbethoxy to obtain woody plant variable kind, wherein it comprises selecting explant, processing, differentially cultivating, inducing and selecting; said explant is woody plant seed, to be cut into 1-2mm stem sections to be planted into culture medium, via differential cultivation, to induce the methanesulfonic acid carbethoxy of callus blast cell. The invention can obtain variable kind in the full expression of plant cell, with high yield. And the processed element is single cell, with small volume and high number, to reduce the cost.

Description

technical field [0001] The invention relates to a method for mutagenizing woody plants by using ethyl methanesulfonate (hereinafter referred to as EMS), in particular a method for using callus embryogenic cells to carry out EMS mutagenesis to obtain woody plant variants. Background technique [0002] EMS is a well-applied mutagen. [0003] The journal "Chinese Herbal Medicine" 2004 Vol.35No.11P.1293-1296 discloses a study on the mutation of periwinkle cell lines induced by EMS. With fast growth and high total indole base content, the variant is an ideal cell line as expected. [0004] The journal "Corn Science" 2003Vol.11 No.3P.74-75, 84 discloses a research on the mutagenic effect of EMS on maize inbred lines. effect, the quality traits of the M2 generation did not change significantly, but the quantitative shapes such as plant height, panicle height, stem circumference, panicle length, and leaf length had a higher mutation frequency. [0005] It can be seen that the mut...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/06A01H4/00C12N5/04
Inventor 苏敏宋学孟陈丽庞瑞华许雄山章振华王凌健张承妹
Owner 上海光兆植物速生技术有限公司
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