Ploidy mutagenesis method of soaking thorn grape seeds with amisulalin

A technology of amisulfulazine and thorn grapes, which is applied in the directions of botanical equipment and methods, application, and plant genetic improvement, etc., to achieve the effects of increasing probability, improving mutagenesis effect, and improving penetration.

Active Publication Date: 2018-02-23
HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC
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  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, there is no successful report on the application of amisulfralin to thorny grapes and induce thorny grape chromosome doubling in China. Sexual induction, providing excellent germplasm resources and new lines for obtaining polyploid new varieties of thorny grapes with large grains, high quality, few seeds or no seeds

Method used

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  • Ploidy mutagenesis method of soaking thorn grape seeds with amisulalin
  • Ploidy mutagenesis method of soaking thorn grape seeds with amisulalin
  • Ploidy mutagenesis method of soaking thorn grape seeds with amisulalin

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The design scheme of the ploidy mutagenesis experiment of amisulalin acting on the germinated thorn grape seeds is shown in Table 1: the experiment was carried out by dipping method. Taking clear water dipping as a contrast, set 3 repetitions, 12 treatments in total; each germination box is filled with 500ml of each treatment solution, soaking 100 thorn grape seeds that have germinated to the dewy stage with roughly the same effect of germination acceleration, and the soaking time is respectively 12h, 24h, 36h. The content of DMF as the organic solvent of amisulfameline in the treatment solution is 0.04%-0.08%.

[0026] The seed immersion test was carried out in a 25°C constant temperature shaker (rotation frequency: 40∽400rpm; shimmy amplitude: Φ25mm).

[0027] After the impregnation treatment of the thorn grape seeds, the seeds were washed with clean water, and sowed in greenhouses respectively, the survival rate was counted, and the growth and development of the tho...

Embodiment 2

[0067] Investigate the ploidy mutagenic effect of the small range of amisulfralin concentration and the short time difference of immersion on the germinated seeds of thorn grape: soak the thorn grape seeds with 0.0025%, 0.003% and 0.0035% concentration of amisulfralin respectively 20h, 24h, 28h (see Table 6), although the mutagenesis rates are all above 46%, they are lower than the optimal conditions (0.0003%, 24h).

[0068] Table 6 The ploidy effect of amisulalin with small variable concentration and short time difference on the ploidy of thorn grape seeds

[0069]

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Abstract

The invention discloses a ploidy mutagenesis method of dinitramine-dipped brier grape seeds. The method comprises the following steps: (1) dissolving dinitramine in dimethylformamide, and diluting via water until the mass concentration of dinitramine is 0.0025%-0.0035% to obtain a dinitramine solution; (2) dipping the brier grape seeds in a white bud emerging stage in the dinitramine solution for 12-36 hours to complete ploidy mutagenesis of the brier grape seeds. Through the ploidy mutagenesis method of dinitramine-dipped brier grape seeds, the brier grape seeds are successively induced to obtain brier grape tetraploids according to a dipping method; the dipping solution is dinitramine; the optimal concentration is about 0.003%; the mutagenesis rate reaches 60% or higher.

Description

technical field [0001] The invention relates to a ploidy mutagenesis method for dipping thorn grape seeds with amisulfamelan. Background technique [0002] The thorny grape (V. davidii. Foex) belongs to the East Asian population, 2n=38. It is one of the main wild grapes in southern my country. Common diseases such as white rot, anthracnose, and black pox have good resistance and strong adaptability. This unique performance has drawn a broad prospect for the pollution-free planting of grapes in the south, and is also a good example for grape moisture-resistant rootstocks and new varieties. Breeding and other aspects provide excellent resources and materials. The new vines, young leaves and fruits of the thorny grape are very colorful, and the ornamental effect and value are higher than that of ordinary grapes. It is an excellent vertical ornamental greening plant in parks and courtyards. The skin color of most thorn grapes is blue-black or blue-purple, and its medicinal effec...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/08
CPCA01H1/08
Inventor 蔡冬元林继华刘芳欧阳英徐一兰邓建平
Owner HUNAN BIOLOGICAL & ELECTROMECHANICAL POLYTECHNIC
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