Rapeseed mutation breeding method

A technology of mutation breeding and rapeseed, which is applied in the direction of botany equipment and methods, application, plant gene improvement, etc., can solve the problems of three types of varieties, and achieve the effect of increasing the probability of trait mutation

Pending Publication Date: 2018-12-21
ZHOUKOU NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the high oil content of rapeseed crops, it can not only be used as raw material for oil extraction of edible oil, but also as raw material for production of industrial oil. Whether it is

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A rapeseed mutation breeding method, comprising the following steps:

[0039] S1, collect the pollen of the male parent and emasculate the female parent

[0040] Collect the pollen of rape male parent Zheda 622 (growth period 230 days, oil content 48.1%) for subsequent use;

[0041] Select female rapeseed parent Zheda 630 (growth period 228 days, oil content 49.2%) with a good growth period and growth period, single plant emasculation, emasculation, pistil bagging isolation, and obtain the female parent of rapeseed to be pollinated;

[0042] S2, mutagenesis treatment

[0043] Remove the anthers from the male parent pollen collected in S1 and irradiate it under a UV lamp. The conditions for the UV lamp irradiation are: place the male parent pollen at 30 cm directly below the 15W UV lamp and irradiate it for 30 seconds to obtain UV mutagenic pollen;

[0044] Soak the ultraviolet mutagenized pollen in the xylene-EMS-paraffin oil solution for 40 minutes at 35°C to obtain ...

Embodiment 2

[0051] A rapeseed mutation breeding method, comprising the following steps:

[0052] S1, collect the pollen of the male parent and emasculate the female parent

[0053] Collect the pollen of rape male parent Zheda 622 (growth period 230 days, oil content 48.1%) for subsequent use;

[0054] Select female rapeseed parent Zheda 630 (growth period 228 days, oil content 49.2%) with a good growth period and growth period, single plant emasculation, emasculation, pistil bagging isolation, and obtain the female parent of rapeseed to be pollinated;

[0055] S2, mutagenesis treatment

[0056] Remove the anthers from the male parent pollen collected in S1 and irradiate it under a UV lamp. The conditions for the UV lamp irradiation are: place the male parent pollen at a place 30 cm directly below the 15W UV lamp and irradiate it for 60 seconds to obtain UV mutagenic pollen;

[0057] Soak the ultraviolet mutagenic pollen in the xylene-EMS-paraffin oil solution for 60 minutes at 40°C to o...

Embodiment 3

[0064] A rapeseed mutation breeding method, comprising the following steps:

[0065] S1, collect the pollen of the male parent and emasculate the female parent

[0066] Collect the pollen of rape male parent Zheda 622 (growth period 230 days, oil content 48.1%) for subsequent use;

[0067] Select female rapeseed parent Zheda 630 (growth period 228 days, oil content 49.2%) with a good growth period and growth period, single plant emasculation, emasculation, pistil bagging isolation, and obtain the female parent of rapeseed to be pollinated;

[0068] S2, mutagenesis treatment

[0069] Remove the anthers from the male parent pollen collected in S1 and irradiate it under a UV lamp. The conditions for the UV lamp irradiation are: place the male parent pollen at a place 30 cm directly below the 15W UV lamp and irradiate it for 45 seconds to obtain UV mutagenic pollen;

[0070] Soak the ultraviolet mutagenized pollen in the xylene-EMS-paraffin oil solution for 40 minutes at 36°C to...

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PUM

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Abstract

The invention belongs to the technical field of rapeseed breeding, and particularly relates to a rapeseed mutation breeding method. The method comprises the steps as follows: collection of paternal pollen, maternal emasculation, mutation treatment, artificial pollination, replanting, and mutants acquisition; according to the invention, xylene, EMS and paraffin oil are adopted to prepare a chemicalmutagen, and UV mutagenesis is used in combination with the chemical mutagen, wherein the chemical mutagen is a composite mutagen prepared by mixing xylene, EMS and paraffin oil in a volume ratio of(0.02-0.03):(0.06-0.07):50. The method provided by the invention is a new breeding method for cultivating new rapeseed varieties, the mutagenesis rate is high, and the screening of new mutant traits is facilitated, and the method lays a foundation for the breeding of new varieties, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the technical field of rapeseed breeding, and in particular relates to a rapeseed mutation breeding method. Background technique [0002] Rapeseed is a variety of cruciferous cabbage, mainly distributed in Anhui, Henan, Sichuan and other places. Rapeseed is rich in nutrients, especially high in vitamin C. Rapeseed flowers have excellent ornamental value. After the flowers wither, rapeseeds can be squeezed for oil, and young rapeseed can also be eaten, so the economic value of rapeseed crops is high. At present, rape varieties are mainly divided into three types: cabbage-type rape, shepherd’s purse-type rape and brassica-type rape. About; shepherd's purse type rape has a tall plant type, slender branches and high positions, small flowers, long growth period, strong drought resistance and barren resistance, and an oil content of about 35%; Brassica napus has a medium plant shape, thick leaves, and flowers Larger, with an oil co...

Claims

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Application Information

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IPC IPC(8): A01H1/02A01H1/06
CPCA01H1/02A01H1/06
Inventor 刘红占李菁菁李俐俐
Owner ZHOUKOU NORMAL UNIV
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