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Creation method of novel stress-resistant germplasm of Ceratostigma minus

A new germplasm, blue snow technology, applied in horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as poor adaptability to high temperature in summer, achieve low mutagenesis frequency, shorten breeding cycle, enrich The effect of germplasm resources

Active Publication Date: 2022-01-21
SICHUAN AGRI UNIV +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Preliminary studies have found that the introduction of wild snowflakes to the city of Chengdu has poor adaptability to the high temperature in summer

Method used

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  • Creation method of novel stress-resistant germplasm of Ceratostigma minus
  • Creation method of novel stress-resistant germplasm of Ceratostigma minus
  • Creation method of novel stress-resistant germplasm of Ceratostigma minus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] A method for creating a stress-resistant new germplasm of Little Blue Snowflake, comprising the following steps:

[0035](1) Select the aseptic explants obtained by the method of subculture and rapid propagation of aseptic tissue culture seedlings of Xiaolan Snowflake (for the method, refer to patent CN107667865 A);

[0036] (2) Add 0-2.0mg·L to the basal medium -1 Pingyangmycin solution;

[0037] (3) Inoculate the aseptic explants of the little blue snowflakes of step (1) into the medium of step (2) and carry out tissue culture (in the embodiment, only selecting the culture temperature is 20°C, the light intensity is 1500Lx, and the light time is 20°C. 8h / d for inspection, other culture conditions defined in the present invention are similar to this condition).

Embodiment 2

[0039] A method for creating a stress-resistant new germplasm of Little Blue Snowflake, comprising the following steps:

[0040] (1) Select the aseptic explants obtained by the method of subculture and rapid propagation of aseptic tissue culture seedlings of Xiaolan Snowflake (for the method, refer to patent CN107667865 A);

[0041] (2) Add 1.5mg·L to the basal medium -1 Pingyangmycin solution;

[0042] (3) the explant of step (1) is inoculated in the substratum of step (2) and carries out mutagenesis culture, and after induction, the aseptic explant of the little blue snowflake is cultivated for 1-7 days and carries out rooting with common substratum , using 35-45°C high temperature treatment for 12-60h to screen high temperature resistant mutants.

experiment example 1

[0044] Using the method of Example 1, according to the scheme in Table 1 below, the effects of different concentrations and different mutagenesis times on the induction efficiency of the small blue snowflake tissue culture seedlings were investigated.

[0045] On the ultra-clean workbench, cut the terminal buds of the well-grown Xiaolan Snowflake tissue culture seedlings with a length of about 0.5-1.0cm as explants, and inoculate them into the non-added PYM (blank control) and the 0.5mg L -1 , 1.0mg·L -1 , 1.5mg·L -1 , 2.0mg·L -1 In the induction medium of PYM, the treatment time has three gradients of 1d, 4d, and 7d, each treatment contains 60 explants, and is repeated 3 times. After finishing the treatment, wash 2-3 times with sterilized phosphate buffer solution (pH=7), insert into the basal medium to continue culturing, count the survival rate of each treatment after 30 days, and then observe the morphological variation of each treatment plant to prevent the induced The...

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Abstract

The invention provides a creation method of a novel stress-resistant germplasm of Ceratostigma minus. The method comprises the following steps: (1) selecting a sterile terminal bud of the Ceratostigma minus as an explant; (2) adding 0-2.0 mg.L<-1> of pingyangmycin into a basic culture medium; (3) inoculating the explant into the culture medium for mutagenesis culture, wherein the culture temperature is 20-28 DEG C, the illumination intensity is 1500-2500 Lx, the illumination time is 8-12 h / d, and the culture time is 1-7 days; and (4) inoculating the induced tissue culture seedlings of the Ceratostigma minus into the basic culture medium, conducting culturing until rooting, and carrying out heat-resistant directional screening at 35-45 DEG C to obtain the novel high-temperature-resistant stress-resistant germplasm of the Ceratostigma minus. According to the method, a large number of high-temperature-resistant mutants are screened out, the mutagenesis rate is high, the problems that in conventional mutagenesis breeding, the mutagenesis frequency is low, and directional breeding cannot be achieved are greatly solved, the breeding period is greatly shortened by 1-2 years, and the purposes of enriching germplasm resources of the Ceratostigma minus and improving breeding are achieved.

Description

technical field [0001] The invention belongs to the technical field of plant breeding, and in particular relates to a method for creating a stress-resistant new germplasm of the little blue snowflake. Background technique [0002] Ceratostigma minus is an upright deciduous shrub or semi-shrub of the genus Ceratostigma Bunge in the family Plumbaginaceae, also known as Zijinbiao, Nine-section Lotus, and Small Hornstigma. The little blue snowflake is a wild species native to our country. It is mostly found in dry shrubs, gravel piles, and sunny places by riversides and roads in dry and hot valleys. It is mainly distributed in western Sichuan and eastern Tibet. Its flower shape is elegant, and its color is blue-purple, which is rare in nature. The average flowering period is about 3 months, and the longest is 6 months. It is a rare wild ornamental flower in garden landscaping. In addition to being used for landscaping, Snowflakes is also a medicinal plant. Folks believe that th...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/008Y02A40/22Y02P60/40
Inventor 高素萍周子祺雷霆周伦理杨丽娟李佳妮
Owner SICHUAN AGRI UNIV
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