A method for mutagenesis and breeding of low-protein and low-phosphorus rice varieties

A low-protein, rice seed technology, applied in the fields of botanical equipment and methods, plant gene improvement, application, etc., can solve the problem of low protein content and other problems, achieve high proportion, improve mutagenesis rate, and good stability.

Active Publication Date: 2019-02-05
安徽绿亿种业有限公司
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AI Technical Summary

Problems solved by technology

At present, my country's research on rice protein is mainly focused on how to increase the total protein or gluten content, and there are not many studies on reducing protein content.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] 1) Select full-grained rice seeds and soak them in distilled water for 10 hours, prepare a 4 mmol / L ethyl methanesulfonate phosphoric acid solution with a phosphate buffer solution with a concentration of 0.04 mol / L, take out the seeds, soak them in it for 6 hours, and rinse them with clean water Rinse 6 times, sow after rinsing with tap water for 1 hour, and harvest the mutagenic M1 generation rice seeds;

[0017] 2) Screening the obtained M1 generation rice seeds, selecting low glutenin and low phosphorus rice seeds, sowing again, performing agronomic trait screening combined with molecular marker-assisted selection at the mature stage, and detecting the glutenin and phosphorus content of M1 single plants, 500 individual plants each confirmed as low gluten genotype and low phosphorus genotype were harvested to obtain M2 generation rice seeds;

[0018] 3) Soak the M2 generation rice seeds in distilled water for 10 hours, prepare a 6 mmol / L sodium azide phosphoric acid ...

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Abstract

The invention provides a method for breeding a low-protein low-phosphorous rice variety by mutation. The method comprises the following steps: mutating rice twice with ethylmethylsulfone phosphoric acid and sodium azide phosphoric acid, detecting and screening rice seeds with low protein and low phosphorous content; hybridizing and backcrossing the rice variety with low protein and low phosphorous content with the parent of hybrid rice; and performing comprehensive observation on agronomic property, protein content, phosphorous content and disease resistance to finally obtaining the low-protein low-phosphorous rice variety. The invention aims at providing the method for breeding a low-protein low-phosphorous rice variety, two chemical mutagens with different mutation mechanisms are used, sodium azide is mainly used for performing base substitution on DNA in duplication, and ethylmethylsulfone is used for alkylating DNA molecules, so that the molecular structure of genes is changed, and the mutation rate can be well promoted; and compound mutation of two solutions has a remarkable effect for breeding low-protein low-phosphorous rice variety.

Description

technical field [0001] The invention belongs to the technical field of rice breeding, and in particular relates to a method for mutation breeding of rice varieties with low protein and low phosphorus. Background technique [0002] Rice is one of the main food crops in my country. The calories, protein and most trace elements in the food of Chinese residents all come from cereals, and its nutritional quality has been paid more and more attention by people. Protein content is one of the most important criteria to measure the nutritional quality of rice, and the protein content of rice is usually 7%-10%. According to the different solubility characteristics, the proteins in rice can be divided into albumin soluble in water, globulin soluble in dilute salt solution, prolamin soluble in alcohol solution and glutenin soluble in dilute acid or dilute alkali solution. Among them, glutenin and globulin are the main storage proteins of rice, accounting for about 80% and 10% of rice p...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/06
CPCA01H1/02A01H1/04A01H1/06
Inventor 张保刚宋永玉崔娟
Owner 安徽绿亿种业有限公司
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