Method for obtaining excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis

A technology for underground stems and clones, which is applied in the field of obtaining excellent clones of bamboo for fiber, can solve the problems of single variety type, not establishing a new set of bamboo germplasm for fiber, and difficulty in variety improvement.

Active Publication Date: 2019-12-17
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The history and current status of bamboo research in China are relatively ahead of other countries in the world, but due to the complexity of the genetic background of bamboo and the particularity of biology, that is, the characteristics of bamboo plants such as uncertainty in flowering and death after flowering, it is difficult to pass Variety improvement by traditional breeding methods
Existing breeding of fine bamboo varieties for fiber

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] Embodiment 1: 1. select the rhizome of Cizhu rhizomes for fibers with 60% cellulose content and 15% lignin content; 2. select internal irradiation mutagen Na 131 1, be respectively mixed with the mutagen solution of 10, 15, 20, 25, 30GBq; 3. choose the chemical mutagen EMS (ethyl methanesulfonate), be mixed with 0.05%, 0.1%, 0.5%, 1.0% respectively , 5.0% mutagen solution; ④ use a stainless steel continuous syringe to fill the different internodes of selected fiber bamboo rhizomes with 10, 15, 20, 25, 30GBq of internal radiation mutagens of different concentrations, and seal them with self-adhesive tape Strictly inject the inlet; ⑤After 30 days, use a stainless steel continuous syringe to fill the different internodes of the selected fiber bamboo rhizomes with 0.05%, 0.1%, 0.5%, 1.0%, 5.0% chemical mutagen solution, and seal it with adhesive tape Strict injection into the entrance; ⑥After 60 days of soil covering, different mutant buds on the underground stems were subj...

Embodiment 2

[0019] Embodiment 2: 1. select the rhizome of Arthia lanceolata for fiber with 40% cellulose content and 25% lignin content; 2. select internal irradiation mutagen Na 131 1, be respectively mixed with the mutagen solution of 10, 15, 20, 25, 30GBq; 3. choose the chemical mutagen EMS (ethyl methanesulfonate), be mixed with 0.05%, 0.1%, 0.5%, 1.0% respectively , 5.0% mutagen solution; ④ use a stainless steel continuous syringe to fill the different internodes of selected fiber bamboo rhizomes with 10, 15, 20, 25, 30GBq of internal radiation mutagens of different concentrations, and seal them with self-adhesive tape Strictly inject the inlet; ⑤ After 40 days, use a stainless steel continuous syringe to fill the different internodes of the selected fiber bamboo rhizome with 0.05%, 0.1%, 0.5%, 1.0%, 5.0% chemical mutagen solution, and seal it with adhesive tape Strict injection into the mouth; ⑥After 90 days of soil covering, different mutant buds on the underground stems undergo mu...

Embodiment 3

[0020] Embodiment 3: 1. select the rhizome of green bamboo for fiber with 50% cellulose content and 20% lignin content; 2. select internal irradiation mutagen Na 131 1, be respectively mixed with the mutagen solution of 10, 15, 20, 25, 30GBq; 3. choose the chemical mutagen EMS (ethyl methanesulfonate), be mixed with 0.05%, 0.1%, 0.5%, 1.0% respectively , 5.0% mutagen solution; ④ use a stainless steel continuous syringe to fill the different internodes of selected fiber bamboo rhizomes with 10, 15, 20, 25, 30GBq of internal radiation mutagens of different concentrations, and seal them with self-adhesive tape Strictly inject the inlet; ⑤After 35 days, use a stainless steel continuous syringe to fill the different internodes of the selected fiber bamboo rhizome with 0.05%, 0.1%, 0.5%, 1.0%, 5.0% chemical mutagen solution, and seal it with adhesive tape Strict injection into the mouth; ⑥ After 75 days of soil covering, different mutant buds on the underground stems undergo multipl...

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Abstract

The invention discloses a method for obtaining an excellent clone through fiber bamboo subterraneous stem in-situ multiple mutagenesis. The method comprises the steps that a subterraneous stem of fiber bamboo is selected; an internal irradiation mutagenic agent is selected to be prepared into mutagenic agent solutions separately; a chemical mutagenic agent ethylmethane sulfonate is selected to beprepared into mutagenic agent solutions separately; a stainless steel continuous syringe is used for filling different segments of the selected subterraneous stem of the fiber bamboo with the internalirradiation mutagenic agent of different concentrations, and injection ports are sealed by non-sticking adhesive tapes; after 30-40 days, the stainless steel continuous syringe is used for filling the different segments of the selected subterraneous stem of the fiber bamboo with the chemical mutagenic agent solution, and the injection ports are sealed by non-sticking adhesive tapes; after earthing is performed for 60-90 days, different mutagenic buds on the segments, subjected to multiple treatment of the internal irradiation mutagenesis and mechanical mutagenesis, of the subterraneous stem are germinated into bamboo, and through screening and underground root dividing propagation, the clonal new germplasm or new species of the fiber bamboo with excellent traits.

Description

technical field [0001] The invention relates to a method for obtaining an excellent clone of bamboo for fiber, and belongs to the technical field of plant breeding. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and even the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the relative shortage of forest resources in our country and the continuous improvement of laws and policies related to forest protection series, as well as the relatively low recycling rate of waste paper in the paper industry chain, the gap in timber resources can only be made up by imports to a large extent, and the country must not Import lumber and wood pulp without spending huge sums of money. However, as a natural plant resource widely distributed in my country, bamboo has the characteristics of wide distribution, strong adaptability, fast growth, early matu...

Claims

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Application Information

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IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 赖金龙张宇罗学刚胡尚连黄艳龙治坚
Owner SOUTHWEAT UNIV OF SCI & TECH
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