A method for inducing flowering hybrid breeding with bamboo stalk buds for fiber
A hybrid breeding and fiber technology, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of low bamboo genetics and breeding
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Embodiment 1
[0016] Embodiment 1: 1. respectively select 40% of cellulose content, 25% of lignin content to use Cizhu and 60% of cellulose content, 15% of lignin content as parents; 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; 100ml of 100μg / ml gibberellin solution, 100ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione were sequentially injected into the internode of the bamboo culms of the two parents at intervals of 5 days Peptide mixed solution 100ml, after each injection, use adhesive tape to seal the injection port; ④ 90 days later, the buds outside the internode septum induced by the bamboo stalks of the two parents were induced to flower, and the hybrid seeds were obtained by artificial pollination, and the seed breeding screen was obtained A new germplasm or new variety of bamboo for fiber with excellent properties.
Embodiment 2
[0017] Embodiment 2: 1. select 60% of cellulose content, the fiber of lignin content 15% respectively to use Cizhu and the fiber of cellulose content 40%, the fiber of lignin content 25% to make parent; 2. select flowering inducer to be combined as 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; Inject 200ml of 100μg / ml gibberellin solution, 200ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione into the internode of the parental bamboo stalk at intervals of 7 days 200ml of mixed solution, after each injection, seal the injection port with adhesive tape; 4. After 60 days, the buds outside the internodes of the bamboo stalk induction treatment of the two parents are induced to flower, and hybrid seeds are obtained through artificial pollination, and the seeds are screened to obtain New bamboo germplasm or new varieties for fib...
Embodiment 3
[0018] Embodiment 3: 1. respectively select 50% of cellulose content, 20% of lignin content fiber to use Cizhu and 45% of cellulose content, 18% of lignin content to make parent; 2. select flowering inducer combination 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; 150ml of 100μg / ml gibberellin solution, 170ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione were injected into the internodes of the bamboo culms of the two parents sequentially at intervals of 6 days Peptide mixed solution 150ml, after each injection, use adhesive tape to seal the injection port; ④ 75 days later, the buds outside the internodes of the bamboo stalks induced by the two parents were induced to flower, and the hybrid seeds were obtained by artificial pollination, and the seeds were screened to obtain A new germplasm or new variety of bamboo for fi...
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