A method for in-situ multiple mutagenesis of bamboo rhizomes used for fiber to obtain excellent clones

Abstract

The invention discloses a method for obtaining excellent clones through multiple mutagenesis of rhizomes of bamboo for fibers, which comprises selecting a rhizome of bamboo for fibers; selecting internal irradiation mutagens to prepare mutagen solutions respectively; and selecting chemical mutagen A Ethyl sulfonate was prepared into mutagen solutions respectively; the different internodes of selected fiber bamboo rhizomes were filled with internal radiation mutagens of different concentrations with a stainless steel continuous syringe, and the injection port was sealed tightly with adhesive tape; 30~ After 40 days, use a stainless steel continuous syringe to fill the different internodes of the selected fiber bamboo rhizomes with a chemical mutagen solution, and seal the injection port with a self-adhesive tape; after 60 to 90 days of covering the soil, the rhizomes are subjected to internal irradiation and chemical mutagenesis. Different mutant buds on the internodes of multiple treatments germinate into bamboos, and new germplasm or new varieties of bamboo clones for fiber with excellent properties are obtained through screening and breeding.

Description

technical field [0001] The invention relates to a method for obtaining an excellent clone of bamboo for fiber, and belongs to the technical field of plant breeding. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and even the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the relative shortage of forest resources in our country and the continuous improvement of laws and policies related to forest protection series, as well as the relatively low recycling rate of waste paper in the paper industry chain, the gap in timber resources can only be made up by imports to a large extent, and the country must not Import lumber and wood pulp without spending a lot of money. However, as a natural plant resource widely distributed in my country, bamboo has the characteristics of wide distribution, strong adaptability, fast growth, early maturity...

AI Technical Summary

Problems solved by technology

The history and current status of bamboo research in China are relatively ahead of other countries in the world, but due to the complexity of the genetic background of bamboo and the particularity of biology, that is, the characteristics of bamboo plants such as uncertainty in flowering and death after flowering, it is difficult to pass Variety improvement by traditional breeding methods

Existing breeding of fine bamboo varieties for fiber is mainly asexual reproduction from natural mutation selection or introduction and domestication from different places, and the variety type is relatively single

In addition, the genetic transformation system of bamboo is still immature, and it is difficult to genetically improve it through genetic engineering.

So far, no modern biotechnology system has been established for the creation and selection of new bamboo germplasm resources for fiber