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Method for stem bud induced flowering type crossbreeding of bamboo poles for fibers

A hybrid breeding and fiber technology, applied in horticultural methods, botanical equipment and methods, chemicals for biological control, etc., can solve the problems of low bamboo genetics and breeding, achieve stable and fast offspring traits, wide range of breeding materials, Simple operation effect

Active Publication Date: 2019-12-24
SOUTHWEAT UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the overall level of bamboo genetics and breeding is still at a low level. It is urgent to formulate and implement strategies and goals for bamboo genetic improvement based on the current research status of bamboo genetics and breeding at home and abroad, so as to promote the development and utilization of bamboo resources for fiber.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Embodiment 1: 1. respectively select 40% of cellulose content, 25% of lignin content to use Cizhu and 60% of cellulose content, 15% of lignin content as parents; 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; 100ml of 100μg / ml gibberellin solution, 100ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione were sequentially injected into the internode of the bamboo culms of the two parents at intervals of 5 days Peptide mixed solution 100ml, after each injection, use adhesive tape to seal the injection port; ④ 90 days later, the buds outside the internode septum induced by the bamboo stalks of the two parents were induced to flower, and the hybrid seeds were obtained by artificial pollination, and the seed breeding screen was obtained A new germplasm or new variety of bamboo for fiber with excellent properties.

Embodiment 2

[0017] Embodiment 2: 1. select 60% of cellulose content, the fiber of lignin content 15% respectively to use Cizhu and the fiber of cellulose content 40%, the fiber of lignin content 25% to make parent; 2. select flowering inducer to be combined as 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; Inject 200ml of 100μg / ml gibberellin solution, 200ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione into the internode of the parental bamboo stalk at intervals of 7 days 200ml of mixed solution, after each injection, seal the injection port with adhesive tape; 4. After 60 days, the buds outside the internodes of the bamboo stalk induction treatment of the two parents are induced to flower, and hybrid seeds are obtained through artificial pollination, and the seeds are screened to obtain New bamboo germplasm or new varieties for fib...

Embodiment 3

[0018] Embodiment 3: 1. respectively select 50% of cellulose content, 20% of lignin content fiber to use Cizhu and 45% of cellulose content, 18% of lignin content to make parent; 2. select flowering inducer combination 100 μg / ml gibberellin solution, 200 μg / ml abscisic acid + 50 μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione mixed solution; 150ml of 100μg / ml gibberellin solution, 170ml of 200μg / ml abscisic acid + 50μg / ml methyl jasmonate mixed solution, 0.1% amylase + 0.05% glutathione were injected into the internodes of the bamboo culms of the two parents sequentially at intervals of 6 days Peptide mixed solution 150ml, after each injection, use adhesive tape to seal the injection port; ④ 75 days later, the buds outside the internodes of the bamboo stalks induced by the two parents were induced to flower, and the hybrid seeds were obtained by artificial pollination, and the seeds were screened to obtain A new germplasm or new variety of bamboo for fi...

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Abstract

The invention discloses a method for stem bud induced flowering type crossbreeding of bamboo poles for fibers. The method includes steps: randomly selecting two types of bamboos for fibers to serve asparents, wherein the cellulose content of the bamboos is 40%-60%, and the lignin content of the bamboos is 15%-25%; selecting a flowering inducer composition which is composed of 100microgram / ml of gibberellin solution, 200microgram / ml of abscisic acid and 50microgram / ml of methyl jasmonate mixed solution and 0.1% amylase and 0.05% glutathione mixed solution; adopting a stainless continuous syringe for sequentially injecting 100-200ml of gibberellin solution, 100-200ml of abscisic acid and methyl jasmonate mixed solution and 100-200ml of amylase and glutathione mixed solution into an internode of the parental bamboo poles for fibers at an interval of 5-7 days, and sealing an injection port with an adhesive sticker each time after injection; 60-90 days later, internode buds obtained in induction treatment of the parental bamboo poles are subjected to flowering induction to obtain crossbreeding seeds through artificial pollination, and the seeds are bred and screened to obtain high-character new germplasms or new species of bamboos for fibers.

Description

technical field [0001] The invention relates to a method for inducing flowering hybrid breeding and belongs to the technical field of plant breeding. Background technique [0002] The pulp and paper industry is one of the important pillar industries of China and even the world economy. With the rapid development of my country's economy, the demand for paper will continue to increase rapidly. Due to the relative shortage of forest resources in our country and the continuous improvement of laws and policies related to forest protection series, as well as the relatively low recycling rate of waste paper in the paper industry chain, the gap in timber resources can only be made up by imports to a large extent, and the country must not Import lumber and wood pulp without spending huge sums of money. However, as a plant natural resource widely distributed in my country, bamboo has the characteristics of wide distribution, strong adaptability, fast growth, early maturity, and high ...

Claims

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Application Information

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IPC IPC(8): A01G7/06A01H1/02A01N63/02A01N45/00A01N43/12A01N37/42A01N37/46A01P21/00
CPCA01G7/06A01H1/02A01N37/42A01N37/46A01N43/12A01N45/00
Inventor 赖金龙张宇罗学刚胡尚连黄艳龙治坚
Owner SOUTHWEAT UNIV OF SCI & TECH
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