Method for breeding new sorghum variety by use of mutation breeding based on diethyl sulfate

A technology of diethyl sulfate and mutagenesis breeding is applied in the agricultural field to achieve the effect of solving the hazard problem and improving the safe use method

Inactive Publication Date: 2016-04-06
SHANXI ACADEMY OF AGRI SCI SORGHUM RES INST
View PDF2 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] There are many kinds of chemical mutagens, such as ethyl methanesulfonate (EMS), colchicine, sodium azide and diethyl sulfate (DES) used in this study. There have been reports on the application of results in crops such as , sorghum, etc. Although there are not many reports on the research and application of EMS, DES is also one of the more commonly used mutagens. There are also many reports on crops such as rice, barley, and oil sunflower. There is no report on the application of DES in sorghum mutation breeding

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for breeding new sorghum variety by use of mutation breeding based on diethyl sulfate
  • Method for breeding new sorghum variety by use of mutation breeding based on diethyl sulfate
  • Method for breeding new sorghum variety by use of mutation breeding based on diethyl sulfate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Embodiment 1: a kind of method that utilizes diethyl sulfate mutagenesis breeding to select new varieties of sorghum, comprises the following steps:

[0016] (1) Indoor diethyl sulfate treatment of sorghum seeds: prepare a 0.3% diethyl sulfate solution with an acid buffer solution of pH = 7 in a fume hood; surface disinfection of sorghum seeds with 0.1% mercury chloride for 15 minutes, aseptic Wash it twice with water, then soak it with the prepared diethyl sulfate solution for 10 hours, then wash it four times with sterile water and set it aside;

[0017] (2) Screen mutants with treated sorghum seeds: put filter paper in a petri dish, autoclave, put the treated sorghum seeds on the filter paper in the sterilized petri dish, soak the filter paper with water, and place in Germinate in an artificial climate box at a temperature of 25°C, and calculate the germination rate, seedling rate, and germination potential. According to the calculated germination rate, seedling rate...

Embodiment 2

[0020] Example 2: A method for breeding new varieties of sorghum by diethyl sulfate mutagenesis, comprising the following steps: (1) processing sorghum seeds indoors with diethyl sulfate: using an acid buffer solution of PH=7 in a fume hood Prepare a diethyl sulfate solution with a concentration of 0.32%. The sorghum seeds are surface-sterilized with 0.1% mercuric chloride for 15 minutes, washed with sterile water for 3 times, then soaked in the prepared diethyl sulfate solution for 12 hours, and then washed with sterile water. After 5 times, it is ready for use; in step (3), the field is planted in 6 rows with a row length of 4m. All the other methods are with the method described in Example 1.

Embodiment 3

[0021] Example 3: A method for breeding new varieties of sorghum using diethyl sulfate mutagenesis, comprising the following steps: (1) processing sorghum seeds with diethyl sulfate indoors: using an acid buffer solution of PH=7 in a fume hood Prepare a diethyl sulfate solution with a concentration of 0.35%; the sorghum seeds are surface-sterilized with 0.1% mercuric chloride for 15 minutes, washed with sterile water for 3 times, then soaked in the prepared diethyl sulfate solution for 14 hours, and then washed with sterile water After 4 times, it is ready for use; in step (3), the field is planted in 3 rows with a row length of 3m. All the other methods are with the method described in Example 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of agriculture and provides a method for breeding new sorghum variety by use of mutation breeding based on diethyl sulfate, so as to solve the problems that existing sorghum variety resource innovation is insufficient, materials for a new breeding technology applied to sorghum breeding are less and the possibility of obtaining a new sorghum variety is low. The method comprises the following steps: treating sorghum seeds indoors by use of diethyl sulfate; screening mutants from the treated sorghum seeds; and performing field cultivation on the screened required mutants so as to screen out mutated descendant materials. The invention establishes a technical method for breeding sorghum through mutation based on diethyl sulfate, so that a safe use method of a toxic chemical DES is further improved, an obvious mutation effect of DES in treatment of sorghum seeds is confirmed, an operation method of treating sorghum seeds by use of the toxic chemical DES is improved, and unwanted hazard problems possibly brought to people can be solved.

Description

technical field [0001] The invention belongs to the technical field of agriculture, and in particular relates to a method for breeding new varieties of sorghum by means of diethyl sulfate mutation breeding. Background technique [0002] Insufficient innovation of sorghum variety resources is the biggest problem facing sorghum breeding at present, and creating resource materials with rich genetic basis is one of the focuses of sorghum breeders' research. Practice has proved that the development and application of new breeding technologies is very important to the innovation of sorghum breeding materials. [0003] Chemical mutagenesis breeding is a new assisted breeding technology developed after selective breeding and cross breeding. Chemical mutagenesis has the characteristics of low cost, convenient use, high mutation frequency, and wide variation range. It is a rapidly developing breeding technology. way. Crop chemical mutation breeding is the artificial use of chemical ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/06
CPCA01H1/06
Inventor 刘勇郝艳芳张微王良群张晓娟杨伟白鸿雁武擘
Owner SHANXI ACADEMY OF AGRI SCI SORGHUM RES INST
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap