Flat screening method of glycerol saccharomyces cerevisiae with low yield

A technology of brewer's yeast and screening method, applied in the field of yeast, can solve the problem of weak reverse reaction of glycerol synthesis, and achieve the effects of improving screening effect, changing blindness and reducing workload

Active Publication Date: 2010-06-16
GUANGXI ZHUJIANG BREWERY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the reverse reaction of glycerol synthesis is weak at too high a glucose concentration

Method used

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  • Flat screening method of glycerol saccharomyces cerevisiae with low yield

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example 1

[0031] Take the following raw materials to make culture medium:

[0032] Glycerol 2%, 2-deoxyglucose 0.05%, Agar 2%,

[0033] Yeast powder 1%, peptone 1%, and the rest made up with distilled water.

[0034] After the medium is sterilized, take 12-15mL and put it into a plate to make the medium evenly distributed.

[0035] Use ethyl methanesulfonate to mutagenize the starting strain, and take the concentration after mutagenesis as 10 -3 ml -1 Spread 0.2mL of bacterial suspension on a flat plate and incubate at 28°C. White colonies can be found on the flat plate. Among them, the strains with larger colonies and neat edges can be regarded as having weak glycerol production ability. Compared with the colonies of the starting strains, the bacteria with colonies larger than the starting strains can be regarded as strains with low glycerol production.

[0036] Pick 10 strains with colonies larger than the starting strain and transfer them to the slant, and cultivate them at a con...

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Abstract

The invention discloses a method for screening out glycerol saccharomyces cerevisiae with low yield by a flat culture medium, comprising the steps of: adopting saccharomyces cerevisiae a start strain; mutating by a chemical mutagen of ethyl methane sulphonate; coating to a designed flat plate; culturing for two to three days; and vaccinating in wheat juice for culturing by shaking a bottle. The method has the advantages that the flat plate adopts glycerol as a unique carbon source; 2-deoxyglucose is added; the strain capable of growing on the flat plate generates lower glycerol quantity; the probability of the glycerol content lower than the glycerol content of the start strain is larger than a conventional screening method; the screening has a certain orientation; and the workload of screening out the strains is reduced.

Description

[0001] Technical field: the invention belongs to the field of yeast, in particular to the breeding and application of beer yeast strains. Background technique: [0002] Glycerin is the main by-product of beer yeast alcohol fermentation. Glycerin has a sweet taste. The glycerin content in general beer easily exceeds the sensory discrimination threshold, which can make the beer have a sweet taste that is not easy to disappear after drinking, making the beer taste unpleasant. In addition, Because glycerin has a strong water absorption effect, it is also one of the important factors affecting the dry mouth after drinking beer. In conclusion, higher glycerol levels in beer can have a detrimental effect on beer. [0003] There are two ways to reduce the content of glycerol in beer: the first way is to control the fermentation process conditions. Unfermented wort is low in glycerol, and the glycerol in beer is mainly produced during fermentation. Therefore, the production of glycer...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/01C12N13/00C12N1/18C12R1/865
Inventor 李红刘杨张五九
Owner GUANGXI ZHUJIANG BREWERY
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