46 results about "Glycerol synthesis" patented technology

The present invention provides for novel metabolic pathways to reduce or eliminate glycerol production and increase product formation. More specifically, the invention provides for a recombinant microorganism comprising a deletion of one or more native enzymes that function to produce glycerol and / or regulate glycerol synthesis and one or more native and / or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source, such as lignocellulose, to a product, such as ethanol, wherein the one or more native and / or heterologous enzymes is activated, upregulated, or downregulated. The invention also provides for a recombinant microorganism comprising one or more heterologous enzymes that function to regulate glycerol synthesis and one or more native and / or heterologous enzymes that function in one or more engineered metabolic pathways to convert a carbohydrate source to ethanol, wherein said one or more native and / or heterologous enzymes is activated, upregulated or downregulated.

The present disclosure relates to recombinant yeast host cells having (i) a first genetic modification for reducing the production of one or more native enzymes that function to produce glycerol or regulating glycerol synthesis and / or allowing the production of an heterologous glucoamylase and (ii) a second genetic modification for reducing the production of one or more native enzymes that function to produce trehalose or regulating trehalose synthesis and / or allowing the expression of an heterologous trehalase. The recombinant yeast host cells can be used to limit the production of (yeast-produced) trehalose (particularly extracellular trehalose) during fermentation and, in some embodiments, can increase the production of a fermentation product (such as, for example, ethanol).

The present invention relates to a yeast cell, in particular a recombinant yeast cell, the cell lacking enzymatic activity needed for the NADH-dependent glycerol synthesis or the cell having a reduced enzymatic activity with respect to the NADH-dependent glycerol synthesis compared to its corresponding wild-type yeast cell, the cell comprising one or more heterologous nucleic acid sequences encoding an NAD+-dependent acetylating acetaldehyde dehydrogenase (EC 1.2.1.10) activity. The invention further relates to the use of a cell according to the invention in the preparation of ethanol.

The present invention relates to a catalyst for producing acrylic acid from glycerin, a preparation method of the catalyst, and an application in synthesizing acrylic acid from glycerol. and an active component, the active component includes at least one technical solution selected from Pt, Re and Rh, which better solves the technical problem and can be used in the industrial production of glycerin to synthesize acrylic acid.

The invention discloses a microalgae triacylglycerol synthesis regulation gene and its encoded protein and application. The gene is derived from Chlamydomonas reinhardtii and is one of the following nucleotide sequences: 1) SEQ ID NO: 1 DNA sequence in the sequence table 2) a polynucleotide encoding the protein sequence of SEQ ID NO: 2 in the sequence listing; 3) a DNA sequence having more than 90% homology with the DNA sequence defined by SEQ ID NO: 1 in the sequence listing and encoding the same functional protein. The encoded protein of the gene is a protein having the amino acid residue sequence shown in SEQ ID NO: 2 in the sequence table, the protein ID is 159133, and the protein is composed of 410 amino acid residues. The CrBbox gene can be applied to change the neutral lipid content of Chlamydomonas reinhardtii, and can be applied to the cultivation of new algal strains with high lipid content.

The invention belongs to the field of biotechnology. The invention relates to a gene with triacylglycerol (TAG) synthesis function and the application of the gene in increasing the TAG content and rationally regulating the TAG saturation of microalgae. The gene is the base sequence shown in SEQ ID NO 1, the base sequence shown in SEQ ID NO2 or has the base sequence shown in SEQ ID NO3; and, respectively with the base sequence shown in SEQ ID NO 1, SEQ ID The base sequence shown in NO 2 or the base sequence shown in SEQ ID NO 3 has more than 95% homology and encodes a DNA sequence of the same biological function protein. The genes of the present invention are three diacylglycerol acyltransferase DGAT genes isolated from Nannochloropsis. The content and saturation of TAG can be rationally regulated by using the obtained gene, in view of the fact that biodiesel, health products and medicines have different demands on the saturation of intracellular triacylglycerol.

The invention relates to a method for producing acrylic acid from glycerin, which mainly solves the problem of low yield of acrylic acid in the process of synthesizing acrylic acid from glycerin in the prior art. The method for producing acrylic acid by using glycerin includes contacting the raw material containing glycerin and the raw material containing oxygen with a catalyst, glycerin A dehydration reaction occurs to generate acrolein, and acrolein is further oxidized to acrylic acid; the catalyst includes a carrier and an active component, and the active component includes at least one technical solution selected from Pt, Re and Rh, preferably The technical problem is solved, and the method can be used in the industrial production of acrylic acid synthesized from glycerin.

The invention provides a method for immobilizing phospholipase D to improve the reaction activity of phosphatidylglycerol synthesis. The method is carried out according to the following steps: step 1, adding the imprinted substrate to the phospholipase D solution for coordination to obtain the enzyme-substrate Complex solution (imprinted enzyme); step 2, adding a precipitating agent to precipitate the enzyme-substrate complex to obtain a mixed solution containing enzyme-substrate complex aggregates; step 3, adding a water-soluble cross-linking agent to the mixed solution after precipitation Perform a cross-linking reaction to obtain cross-linked enzyme-substrate complex aggregates; step 4, wash the cross-linked enzyme-substrate complex aggregates, and obtain cross-linked imprinted phospholipase D aggregates after removing the substrate. In the method of the present invention, after the imprinting action between the phospholipase D molecule and the substrate molecule is completed, the precipitation method is used to "fossilize" the "superactivated" structure of the enzyme molecule, and then add an appropriate water-soluble cross-linking agent to carry out cross-linking of the enzyme molecule , achieving intermolecular cross-linking and intramolecular cross-linking of "superactivated" structural enzyme molecules to form cross-linked imprinted phospholipase D aggregates.

The invention is suitable for the field of biomass energy, and provides a method for producing biodiesel by means of a lignocellulose material. The method comprises the steps that pretreatment is conducted on the lignocellulose material through an alkali-glycerin-methanol mixed solution produced in the process that the biodiesel is prepared by conducting single-step base catalysis transesterification on microbial lipid; after in-situ evaporation is conducted on the raw material, and methanol is subjected to condensation recovery, water is added to reach the proper solid-to-liquid ratio, after acid neutralization is conducted, an appropriate number of enzymes are added for hydrolysis, solid-liquid separation is conducted, and hydrolysate is obtained; oleaginous yeast is introduced and synthesizes lipid by means of carbohydrates and glycerin in the hydrolysate; microbial thalli containing oil are collected, intracellular lipid is extracted, the biodiesel is prepared through single-step base catalysis transesterification, n-hexane is added for extraction, the biodiesel is obtained on the upper layer, the alkali-glycerin-methanol solution is obtained on the lower layer, and the alkali-glycerin-methanol solution is recycled for pretreating lignocellulose. According to the method for producing the biodiesel by means of the lignocellulose material, strong alkali, by-product glycerin and methanol are used for pretreating the lignocellulose; meanwhile, the glycerin further serves as a carbon source of oleaginous microorganisms to be subjected to in-situ integration recycling, the cost is greatly reduced, and the significant economic benefit is achieved.