A kind of method utilizing lignocellulosic raw material to produce biodiesel

A technology for lignocellulose and biodiesel, which is applied in the fields of biofuel, fat production, fatty acid production, etc., can solve the problems of low oil yield, high production cost, difficult resource utilization of by-products, etc., so as to increase yield and reduce reagent cost. , Improve the effect of C/N ratio

Active Publication Date: 2020-07-24
江西碳金科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] In view of the above problems, the object of the present invention is to provide a method for producing biodiesel from lignocellulosic raw materials, aiming to solve the outstanding problems of high production cost, low oil yield and difficult resource utilization of by-products in existing methods

Method used

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  • A kind of method utilizing lignocellulosic raw material to produce biodiesel
  • A kind of method utilizing lignocellulosic raw material to produce biodiesel

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 1) Preparation of corn stover pretreatment and hydrolysis solution: Weigh 50g corn stover that has passed through a 40-mesh sieve, add 200g KOH-glycerol-methanol solution (KOH 2g, glycerol 4g, methanol 194g), the solid-liquid ratio is 25% (w / w) ), treated in a pretreatment tank at 180°C for 15min; in situ evaporation and condensation to recover methanol, add water to adjust the solid-liquid ratio to 10% (w / w), add H 2 SO 4 Adjust the pH to 4.8, add cellulase 15FPU / g, β-glucosidase 30CBU / g and xylanase 100U / g, hydrolyze in a water bath shaker at 50°C and 200rpm for 48h to obtain a hydrolyzate; the hydrolyzate is boiled for 10min , vacuum filtration to obtain the hydrolysis supernatant, wherein the glucose, xylose and glycerol concentrations are 30.1g / L, 18.5g / L and 15.8g / L respectively, the C / N ratio is 72, the pH is adjusted to 6.0 and then quenched at 121°C Bacteria for standby after 20min;

[0044] 2) Add Cryptococcus curvatus ATCC 20509 (purchased from the American...

Embodiment 2

[0049] 1) Preparation of straw pretreatment and hydrolyzate: Weigh 20g of straw that has passed through a 20-mesh sieve, add 40g of KOH-glycerol-methanol solution (KOH 0.2g, glycerol 2g, methanol 37.8g), and the solid-to-liquid ratio is 50% (w / w), treated in a pretreatment tank at 80°C for 2 hours; in-situ evaporation to remove methanol, adding water to adjust the solid-liquid ratio to 5% (w / w), adding H 2 SO 4 Adjust the pH to 4.5, add cellulase 20FPU / g, β-glucosidase 40CBU / g and xylanase 100U / g, hydrolyze in a water bath shaker at 50°C and 200rpm for 72h to obtain a hydrolyzate; the hydrolyzate is boiled for 10min , vacuum filtration to obtain the hydrolysis supernatant, wherein the glucose, xylose and glycerol concentrations are 14.5g / L, 8.4g / L and 4.8g / L respectively, the C / N ratio is 62, and the pH is adjusted to 5.5 and then quenched at 121°C. Bacteria for use after 15min;

[0050] 2) Insert Rhodosporidium toruloides AS 2.1389 (purchased from China Microbial Culture C...

Embodiment 3

[0055] 1) Preparation of cotton stalk pretreatment and hydrolysis solution: Weigh 10 g of cotton stalk that has passed through a 40-mesh sieve, add 100 g of NaOH-glycerol-methanol solution (NaOH 2 g, glycerol 2.5 g, methanol 90 g), and the solid-to-liquid ratio is 10% (w / w), treated in a pretreatment tank at 140 °C for 2 h; in situ evaporated to remove methanol, added water to adjust the solid-liquid ratio to 25% (w / w), added H 3 PO 4 Adjust the pH to 5.5, add cellulase 15FPU / g, β-glucosidase 30CBU / g and xylanase 100U / g, hydrolyze in a water bath shaker at 50°C and 200rpm for 72h to obtain a hydrolyzate; the hydrolyzate is boiled for 10min , vacuum filtration to obtain the hydrolysis supernatant, wherein the glucose, xylose and glycerol concentrations are 81.1g / L, 54.5g / L and 60.8g / L respectively, the C / N ratio is 102, and the pH is adjusted to 6.0 and then quenched at 121°C. Bacteria for use after 15min;

[0056] 2) Lipomyces starkeyi AS 2.1560 (purchased from China Genera...

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Abstract

The invention is suitable for the field of biomass energy, and provides a method for producing biodiesel by means of a lignocellulose material. The method comprises the steps that pretreatment is conducted on the lignocellulose material through an alkali-glycerin-methanol mixed solution produced in the process that the biodiesel is prepared by conducting single-step base catalysis transesterification on microbial lipid; after in-situ evaporation is conducted on the raw material, and methanol is subjected to condensation recovery, water is added to reach the proper solid-to-liquid ratio, after acid neutralization is conducted, an appropriate number of enzymes are added for hydrolysis, solid-liquid separation is conducted, and hydrolysate is obtained; oleaginous yeast is introduced and synthesizes lipid by means of carbohydrates and glycerin in the hydrolysate; microbial thalli containing oil are collected, intracellular lipid is extracted, the biodiesel is prepared through single-step base catalysis transesterification, n-hexane is added for extraction, the biodiesel is obtained on the upper layer, the alkali-glycerin-methanol solution is obtained on the lower layer, and the alkali-glycerin-methanol solution is recycled for pretreating lignocellulose. According to the method for producing the biodiesel by means of the lignocellulose material, strong alkali, by-product glycerin and methanol are used for pretreating the lignocellulose; meanwhile, the glycerin further serves as a carbon source of oleaginous microorganisms to be subjected to in-situ integration recycling, the cost is greatly reduced, and the significant economic benefit is achieved.

Description

technical field [0001] The invention belongs to the technical field of biorefinery and bioenergy, and in particular relates to a method for producing biodiesel by utilizing lignocellulose raw materials. Background technique [0002] Under certain conditions, some microorganisms in nature can use carbohydrates, hydrocarbons, carbon dioxide, etc. as carbon sources to synthesize and store a large amount of oil in the body. w, where w / w represents the mass ratio, the same below) microorganisms are called oil-producing microorganisms. Some oil-producing microorganisms even accumulate more than 70% of their dry cell weight. Microbial oils, especially oils produced by oleaginous fungi, the main component is triglycerides, the fatty acid composition is similar to that of commercial animal and vegetable oils, with C 14 -C 22 Long-chain fatty acids predominate. Compared with animal and vegetable oils, microbial oils have a short production cycle, are not limited by seasons and cli...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C11C3/04C11C1/08C11B1/02C10L1/02
CPCC10L1/02C10L2290/543C11B1/025C11C1/08C11C3/04C12P7/649Y02E50/10
Inventor 龚志伟赵觅
Owner 江西碳金科技有限公司
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