Breeding method for three-line ultra-small-embryo hybrid rice

A hybrid rice and seed technology is applied in the field of selection and breeding of three-line extra-small embryo hybrid rice, which can solve the problem of low yield and achieve the effects of increasing thousand-grain weight, high breeding efficiency and increasing farmers' income.

Inactive Publication Date: 2012-08-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In order to increase the rate of rice polishing, people start from all aspects of raw materi

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] Take 700 grams each of rice three-line restorer line FR316 and maintainer line T55B seeds, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and treat them with a mixture of chemical mutagen manganese sulfate and diethyl sulfate (DES) 6 hours; after treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks were removed to form brown rice, and the extra-small-embryo mutant brown rice kernels were visually selected from the brown rice; the extra-small-embryo mutant brown rice kernels of FR316 and T55B were planted respectively, and FR316 and T55B were used as controls, and the agronomic traits of all mutants were evaluated at the heading and flowering stage. The FR316 ultra-small embryo mutant with similar agronomic traits to FR316 and good flowering and loose powder traits was selected as the three-line ultra-small embryo resto...

Embodiment 2

[0017] Take 700 grams of the seeds of the three-line restorer line Shuhui 527 and the maintainer line D62B each, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and mix with chemical mutagen manganese sulfate and diethyl sulfate (DES) solution treatment for 4 hours; after the treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks were removed to form brown rice, and the extra-small embryo mutant brown rice grains were visually selected from the brown rice; the extra-small-embryo mutant brown rice grains of Shuhui 527 and D62B were planted respectively, and Shuhui 527 and D62B were used as controls, and all mutant brown rice grains were selected at the heading and flowering stage. The agronomic traits and flowering and loose powder traits of the mutants were investigated, and the super small embryo mutant of Shuhui 527 with simil...

Embodiment 3

[0019] Take 700 grams each of the rice three-line restorer line Minghui 86 and maintainer line II-32B seeds, soak the seeds for 24 hours, accelerate germination at 30°C for 18-24 hours until the seed buds are white, and use chemical mutagen manganese sulfate and diethyl sulfate (DES). ) mixed solution for 5 hours; after the treatment, the seeds were rinsed twice with water; 0 ), Mixed M 0 Plant outgrown plants (M 1 ) on the seed (M 2 ); M 2 After drying, the husks are removed to form brown rice, and the extra-small embryo mutant brown rice grains are visually selected from the brown rice; Minghui 86 and II-32B extra-small-embryo mutant brown rice kernels are planted respectively, and Minghui 86 and II-32B are used as controls, and flowering occurs at heading The agronomic traits and flowering and pollen-losing traits of all mutants were investigated in the early stage, and the super-small embryo mutant of Minghui 86 with similar agronomic traits to Minghui 86 and good flow...

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Abstract

The invention relates to a breeding method for three-line ultra-small-embryo hybrid rice, which belongs to the breeding technical field of special paddy rice. According the characteristics that the properties of ultra-small embryos of paddy rice are controlled by a pair of recessive genes, chemical mutagens such as manganese sulfate and diethyl sulfate (DES) are used for collectively processing sprouted seeds of a restorer line and a maintainer line of rice three lines to acquire ultra-small embryo property mutants, the maintainer-line ultra-small-embryo mutant is hybridized and backcrossed with a corresponding sterile line to breed the three-line ultra-small-embryo sterile line, and then the three-line ultra-small-embryo sterile are matched into the three-line ultra-small-embryo hybrid rice. According to the breeding method, the mutation frequency of the ultra-small-embryo property reaches 0.005 to 0.01 percent, the embryo weight of the bred three-line ultra-small-embryo hybrid rice only accounts for 1 to 1.3 percent of a brown rice grain, the size of the bred three-line ultra-small-embryo hybrid rice is only one third to half of that of an ordinary rice embryo, and the head rice rate can be improved by 1 to 2 percent compared with ordinary rice.

Description

technical field [0001] The invention specifically relates to a method for breeding three-line extra-small-embryo hybrid rice, and belongs to the technical field of special rice breeding. Background technique [0002] The brown rice of rice is mainly composed of endosperm and embryo. The main component of endosperm is starch, and the embryo contains more nutrients such as protein, fat and amino acid. Therefore, some breeders increase the nutrient content of brown rice by increasing the size of the embryo, such as giant embryo rice. However, to eat the nutrients in the germ, you must eat brown rice, because the germ and seed coat have been removed (remaining in the bran) during the process of milling brown rice into polished rice, and the polished rice we eat does not contain germ. Brown rice is difficult to boil because of poor taste, and people do not like to eat it. The polished rice we eat is removed during the rice milling process, because the husk, seed coat and embryo...

Claims

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Application Information

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IPC IPC(8): A01H1/06A01H1/02A01H1/04
Inventor 章清杞陈幼玉
Owner FUJIAN AGRI & FORESTRY UNIV
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