Creation method of salt-resistant corn

Abstract

The invention discloses a creation method of a salt-resistant corn. The method includes the following steps: (1) with seedlings sterilely germinated from excellent selfing line seeds as a material, cutting stem tips and performing in-vitro cultivation to inductively generate multiple shoot blocks; (2) performing mutation to the multiple shoot blocks with a chemical mutagen, and moving the multiple shoot blocks onto a culture medium with addition of sodium chloride after recovery cultivation to perform salt-resistant screening; (3) transferring survival tissue blocks to a differential culture medium to induce growth of small seedlings; (4) performing regenerated plant selfing or backcross with a donor selfing line plant to grow seeds to obtain progenies; (5) performing salt-resistant screening in the germination stage of the seeds and seedling period salt resistance detection to the progenies to select the plant strains being excellent in salt resistance; (6) planting the selected plant strains in saline and alkaline land, observing the agricultural characters of the strains and detecting combining ability to culture an excellent salt-resistant selfing line, and further culturing a salt-resistant high-yield hybrid. The method greatly increases the breeding speed of the salt-resistant selfing line, and has important significance in salt-resistant and drought-resistant breeding and agriculture production of corns.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, and in particular relates to a method for creating salt-tolerant corn through the combined techniques of bud mass induction, mutagenesis and directional screening. Background technique [0002] Soil salinization is a global problem currently restricting agricultural production, and 20% of the world's arable land is threatened by salt damage. According to incomplete statistics from UNESCO and FAO, the area of ​​saline-alkali land in the world is 954 million hectares. There are 26 million hectares of saline-alkali land in my country, and 20 million hectares of salinized land have not been effectively developed and utilized. Salinization is also a major environmental factor affecting crop yields. Of the 1.8 billion mu of arable land in my country, there are more than 100 million mu of saline-alkali land, and about 1 billion mu of medium- and low-yield fields, most of which are also ...

AI Technical Summary

Problems solved by technology

Because maize callus or cells have lost or poor ability to regenerate plants after a long period of salt tolerance selection, even if regenerated plants are obtained, it is difficult to self-fertilize due to abnormal plant growth and shape