Creation method of salt-resistant corn

A corn and salt-tolerant technology, applied in the field of bioengineering breeding of crops, can solve problems such as abnormal plant growth and development, difficulty in self-fertilization, loss of ability to regenerate plants, etc., and achieve the effect of improving the speed of breeding.

Inactive Publication Date: 2016-01-13
山东连发农业科技有限公司
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AI Technical Summary

Problems solved by technology

Because maize callus or cells have lost or poor ability to regenerate plants after a long period of salt tolerance selection, even if regenerated plants are obtained, it is difficult to self-fertilize due to abnormal plant growth and shape

Method used

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Examples

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Embodiment 1

[0033]Embodiment 1 Breeding of Chang 7-2 salt-tolerant inbred line

[0034] Chang 7-2, the backbone inbred line of maize in my country, is sensitive to salt stress. Zhengdan 958, a single-cross hybrid made from it as the male parent, has strong adaptability and high yield, but its yield is not high in saline-alkali soil. It is important to improve the salt tolerance of Chang 7-2.

[0035] First, ears were harvested from healthy Chang 7-2 plants, which were protected from microbial and soil contamination during drying, threshing and storage.

[0036] The seeds of Chang 7-2 were soaked in 70% ethanol for 8 minutes, then soaked in 0.1% mercuric chloride for 10 minutes, and then washed 3-4 times with sterile water. After sterilization, the seeds were germinated in a sterile Erlenmeyer flask, and a small amount (35 ml / 250 ml Erlenmeyer flask) of sterile water was put into the bottle, sealed and placed under dark conditions (25° C.) for 2 days. After germination (lubai), the seeds...

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Abstract

The invention discloses a creation method of a salt-resistant corn. The method includes the following steps: (1) with seedlings sterilely germinated from excellent selfing line seeds as a material, cutting stem tips and performing in-vitro cultivation to inductively generate multiple shoot blocks; (2) performing mutation to the multiple shoot blocks with a chemical mutagen, and moving the multiple shoot blocks onto a culture medium with addition of sodium chloride after recovery cultivation to perform salt-resistant screening; (3) transferring survival tissue blocks to a differential culture medium to induce growth of small seedlings; (4) performing regenerated plant selfing or backcross with a donor selfing line plant to grow seeds to obtain progenies; (5) performing salt-resistant screening in the germination stage of the seeds and seedling period salt resistance detection to the progenies to select the plant strains being excellent in salt resistance; (6) planting the selected plant strains in saline and alkaline land, observing the agricultural characters of the strains and detecting combining ability to culture an excellent salt-resistant selfing line, and further culturing a salt-resistant high-yield hybrid. The method greatly increases the breeding speed of the salt-resistant selfing line, and has important significance in salt-resistant and drought-resistant breeding and agriculture production of corns.

Description

technical field [0001] The invention belongs to the field of bioengineering breeding of crops, and in particular relates to a method for creating salt-tolerant corn through the combined techniques of bud mass induction, mutagenesis and directional screening. Background technique [0002] Soil salinization is a global problem currently restricting agricultural production, and 20% of the world's arable land is threatened by salt damage. According to incomplete statistics from UNESCO and FAO, the area of ​​saline-alkali land in the world is 954 million hectares. There are 26 million hectares of saline-alkali land in my country, and 20 million hectares of salinized land have not been effectively developed and utilized. Salinization is also a major environmental factor affecting crop yields. Of the 1.8 billion mu of arable land in my country, there are more than 100 million mu of saline-alkali land, and about 1 billion mu of medium- and low-yield fields, most of which are also ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04A01H1/06A01H4/00
Inventor 李朝霞靳传娣徐绍栋
Owner 山东连发农业科技有限公司
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