Cordyceps sinensis mutant strain for production of Cordyceps sinensis mycelia

A technology of Cordyceps sinensis hyphae and Cordyceps sinensis, applied in the field of new strains of Cordyceps sinensis

Inactive Publication Date: 2016-05-18
JIANGSU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] The first inventor of this patent, Liu Weimin, once supervised the master student Chen Jing to complete the master's thesis "Research on High-value Cordyceps Liquid Fermentation and Utilization of Rice Bran to Produce Polysaccharides". Liquid fermentation, but further research is needed in terms of efficiency

Method used

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  • Cordyceps sinensis mutant strain for production of Cordyceps sinensis mycelia
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  • Cordyceps sinensis mutant strain for production of Cordyceps sinensis mycelia

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Embodiment

[0051] Cordyceps sinensis strain adopts Cordyceps sinensis CCTCCM2015064. The amount of rice bran used is 3.6g / 100mL medium, the amount of bran used is 4.8g / 100mL medium, and KH 2 PO 4 0.27g / 100mL, MgSO 4‘ 7H 2 O0.27g / 100mL, cysteine ​​hydrochloride 50mg / 100mL, sodium copper chlorophyllin 10mg / 100mL, pH natural, add water to the required volume, sterilize at 121°C for 30min, use it as a medium for fermentation, shake bottle The sample volume was 40%, the inoculum volume was 10%, and the culture temperature was 20 o C, rotating speed 180r / min, culture time 5d. The following steps (1) to (7) are carried out: (1) The mycelium containing a small amount of rice bran obtained from liquid culture is separated by centrifugation or centrifugal filtration, washed twice with water, and centrifuged to remove water; ( 2) Freeze-dry the obtained wet mycelium to obtain the finished product of mycelium powder; (3) After filtering and removing slag from the obtained fermented liquid, take...

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Abstract

The invention discloses a Cordyceps sinensis mutant strain for production of Cordyceps sinensis mycelia and relates to the field of application of a food microorganism technology. The mutant strain is collected in CCTCC (China Center for Type Culture Collection) in Wuhan, China on Jan. 23rd, 2015 with the number of CCTCC M 2015064 and the recommended Latin name of Cordyceps sinensis CCTCC M 2015064. In order to avoid use of toxic and harmful chemical mutagens and a genetic engineering method which is not recognized by the public in the food field, an ultraviolet physical mutagenesis technology is considered to be simple and easy, so that the ultraviolet mutagenesis method is adopted for mutagenesis, excellent characters of the novel Cordyceps sinensis strain are guaranteed through a series of screening methods in aspects of stability, hereditary and the like, and a strain basis for obtaining better-quality Cordyceps sinensis mycelium raw material, which can be used for producing healthy food, for industrial efficient fermentation of low-cost and novel rice bran and wheat bran complete liquid culture media to which cysteine hydrochloride and sodium copper chlorophyllin are added.

Description

technical field [0001] The invention relates to the application field of food microorganism technology, in particular to a new strain of Cordyceps sinensis obtained by mutagenesis that can be used to ferment liquid rice bran and bran whole material new medium and produce Cordyceps sinensis mycelium raw material. Background technique [0002] Medicinal and edible fungi have a long history of use in China and a wide range of users. Modern science reveals that the mycelium, fruiting bodies or spores of commonly used medicinal and edible fungi such as Cordyceps sinensis, Hericium erinaceus, Ganoderma lucidum, Grifola frondosa, etc. can produce amino acids, proteins, vitamins, polysaccharides, nucleosides, Various active ingredients such as flavonoids and antibiotics, or enriched with various trace elements such as selenium and zinc, have various functions such as improving human immunity, anti-tumor, enhancing liver function, and anti-oxidation. Because of this, the enthusiasm ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12R1/645
CPCC12N1/145C12R2001/645
Inventor 刘伟民王雪梅王冲之李婷婷周彬张凤霞狄新园
Owner JIANGSU UNIV
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