Mutagenesis method for inducing plant variation and application thereof

A technology for inducing plants and mutations, applied in botany equipment and methods, applications, plant genetic improvement, etc., to achieve the effect of increasing crop yield

Inactive Publication Date: 2018-11-27
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are many mutant libraries constructed from cultivated soybeans, but there are no reports on the construction of mutant libraries using wild soybeans as materials.

Method used

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  • Mutagenesis method for inducing plant variation and application thereof
  • Mutagenesis method for inducing plant variation and application thereof
  • Mutagenesis method for inducing plant variation and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] A kind of mutagenesis method that induces plant to produce variation, induces plant genetic gene to mutate by chemical mutagen, and chemical mutagen is ethyl methanesulfonate (EMS), and its molecular formula CH 3 SO 2 OC 2 h 5 , whose structure is

[0039] The mutagenic mechanism of ethyl methane sulfonate (EMS) is that the oxygen atom position of guanine in the DNA double strand is alkylated by EMS. During DNA replication, the alkylated guanine can be combined with thymine Pairing, resulting in base substitution, changing G:C to T:A. Compared with other mutagenesis methods, EMS mutagenesis has the following characteristics:

[0040] (1) Higher mutation frequency and less chromosomal aberration: EMS mutagenesis mainly produces more point mutations on DNA molecules, which can cause less chromosomal damage than chromosomal breakage and distortion, and can target a particular crop. properties are improved.

[0041] (2) Light damage to treated materials: Compared wi...

Embodiment 2

[0053] Other content is as in the embodiment, wherein the chemical mutagen mutagenesis treatment includes the following steps:

[0054] (1) Preparation of phosphate buffer: take raw materials according to the ratio of Na2HPO4·12H2O:NaH2PO4 to 4.2. Dilute to 100mL with water, pH7.2, phosphate buffer molar concentration is 0.1mol / L.

[0055] (2) Seed soaking: Soak the seeds with phosphate buffer for 7 hours, rinse and put the seeds into the Erlenmeyer flask;

[0056] (3) Preparation of chemical mutagen solution: dissolve the chemical mutagen in 70% alcohol, add phosphate buffer, stir and mix well to obtain the product.

[0057] (4) Pour the prepared chemical mutagen solution into the Erlenmeyer flask, seal it, soak the seeds for 26 hours, and shake slowly during the period;

[0058] (5) Rinse with running water for 1.2 hours to remove residual chemical mutagen solution;

[0059] (6) Carry out subsequent steps of seed imbibition, germination, and transplanting.

Embodiment 3

[0061] Other content is as in the embodiment, wherein the chemical mutagen mutagenesis treatment includes the following steps:

[0062] (1) Preparation of phosphate buffer: take raw materials according to the ratio of Na2HPO4·12H2O:NaH2PO4 to 4. Dilute to 100mL with water, pH7, and the molar concentration of phosphate buffer is 0.1mol / L.

[0063] (2) Seed soaking: Soak the seeds in phosphate buffer solution for 6 hours, rinse and put the seeds into the Erlenmeyer flask;

[0064] (3) Preparation of chemical mutagen solution: dissolve the chemical mutagen in 70% alcohol, add phosphate buffer, stir and mix well to obtain the product.

[0065] (4) Pour the prepared chemical mutagen solution into the Erlenmeyer flask, seal it, soak the seeds for 24 hours, and shake slowly during the period;

[0066] (5) Rinse with running water for 1 hour to remove residual chemical mutagen solution;

[0067] (6) Carry out subsequent steps of seed imbibition, germination, and transplanting.

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Abstract

The invention belongs to the technical field of plant mutation breeding, and provides a mutagenesis method for inducing plant variation and an application thereof. The method is characterized in thata chemical mutagen is used to induce genetic variation in plants, wherein the chemical mutagen is ethyl methyl sulfonate with the molecular formula being CH3SO2OC2H5. Characters of soybeans are changed by inducing the genetic variation in the soybeans, and a soybean mutant library is constructed. Furthermore, shade-tolerant mutants are screened from the mutants. Finally, the mutants are applied toa plant shade avoidance reaction, which can reduce the sensitivity of shade response of low and medium stem crops in intercropping, thereby increasing the yield of the low stem crops in the intercropping. The method can produce novel varieties of plants that meets human production needs or have beneficial effects on other organisms and ecological environment, and makes the plants rapidly developin the direction that benefits human beings.

Description

technical field [0001] The invention belongs to the field of plant mutation breeding, in particular to a mutagenesis method for inducing variation in soybeans and an application thereof. Background technique [0002] The intercropping soybean planting mode is of great significance to increase the soybean yield and promote the sustainable development of agriculture in my country. . However, due to intercropping with high-stalk crops, the shady environment formed by high-stalk crops makes the photosynthetic product accumulation of soybean insufficient and easy to lodging. Therefore, avoiding shade and lodging has become one of the important factors restricting the yield of intercropping and intercropping soybeans. At present, soybean varieties suitable for intercropping and intercropping are very scarce, which hinders the development of southern soybeans to a certain extent. On the other hand, wild soybeans are native to China. Compared with cultivated soybeans, they have r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/00A01H1/06
CPCA01C1/00A01H1/06
Inventor 杜俊波李振宇孙孟园白云黎艳蒋亨珂孙歆余靓刘江舒凯刘卫国杨峰王小春雍太文杨文钰
Owner SICHUAN AGRI UNIV
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