Phytoseiulus persimilis sperm acquisition and slide specimen preparation method

Abstract

The invention relates to a phytoseiulus persimilis sperm acquisition and slide specimen preparation method. The method is characterized in that the method for preparing a slide specimen by acquiring spermatophore from gnathosoma of male phytoseiulus persimilis in mating includes acquisition of male and female phytoseiulus persimilis in mating, fixing the male and female phytoseiulus persimilis, separation of the male and female phytoseiulus persimilis, spermatophore acquisition, sperm acquisition and semen slide specimen preparation. Spermatophore acquisition provides raw materials for phytoseiulus persimilis sperm slide specimen preparation and research, and semen slide specimen preparation provides references for knowing morphology of phytoseiulus persimilis sperms and revealing phytoseiulus persimilis reproduction principles.

Description

technical field [0001] The invention relates to a method for acquiring sperm of Phytoseiid mite chile and making a slide specimen, and belongs to the field of basic biological research of Phytoseiid mite chile. Background technique [0002] Phytoseiid mite is a dioecious animal, which requires mating and fertilization of male and female mites before laying eggs and producing offspring. [0003] The existing information on the mating of male and female phytoseiid mites to form fertilized eggs is that the sperm of the male mites are stored in the spermatozoa before entering the female mites, and the female mites stuff the spermatozoa into the mating holes of the female mites (Reference: Liang Lairong , 1979, Plant Protection, How do mites live), the sperm is slowly released from the seminal vesicle, transferred to the fertilizing vesicle and combined with the egg to form a fertilized egg. However, there is no report on how the sperm of the male Phytoseiid mite enter the femal...

AI Technical Summary

Problems solved by technology

[0004] However, because the individuals of Phytoseioid mites are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. , and the size of the spermatozoa is only a dozen microns and is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain spermatozoa, and it is even more difficult to make spermatozoon slide specimens. It is necessary to develop a method to obtain phytoseioid semen and to make phytoseiillus semen slide specimens.