Method for obtaining and preserving spermatophores of Amblyseius tsugawai

A technology of Amblyseius and spermatozoa, which is applied in animal husbandry and other fields, can solve the problems of not getting spermatozoa and being difficult to see

Inactive Publication Date: 2018-01-12
山东首伯农生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, because the individuals of A. jinsichuan are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0012] Example The method for obtaining the essence of Amblyseius jinchuan

[0013] This method is a method of artificially obtaining and picking out the spermatozoa from the jaws of the male mite of A. jinsichuanensis during mating, including the acquisition of the male and female mites during mating, the typing of the male and female mites, the separation of the male and female mites, the acquisition of the spermatocyst and Save 4 parts, all operations are carried out under a microscope except for the determination of the male and female mites during mating. The specific operations are:

[0014] (1) Acquisition of male and female mites during mating: Randomly select a pair of male and female mites from the rearing population of A. The time may be longer than 10 minutes, and it is necessary to observe whether the mating male and female mites mate to obtain the mating male and female mites;

[0015] (2) The stereotyping of male and female mites during mating: Within 0-120 min...

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PUM

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Abstract

The invention relates to a method for obtaining and preserving spermatophores of Amblyseius tsugawai. The method is characterized in that spermatophores are obtained and picked from gnathosoma of maleAmblyseius tsugawai in the mating process, the method includes the steps of acquisition of females and males in the mating process, setting of the females and the males, separation of the females andthe males and acquisition and preservation of the spermatophores, and all operations are carried out under the microscope except setting of the females and the males in the mating process. The spermatophores of the male Amblyseius tsugawai can be obtained by means of the extremely easy and smart method, and acquisition of the spermatophores can provide a material source for research of the spermsof Amblyseius tsugawai; the method can also provide reference for research of the spermatophores of the other predatory mites.

Description

technical field [0001] The invention relates to a method for obtaining and preserving the essence package of Amblyseius jinchuan, which belongs to the field of basic biological research of amblyseius jinchuan. Background technique [0002] Amblyseius jinchuanensis is a dioecious animal, and only after the mating and fertilization of male and female mites can it lay eggs and breed offspring, and both male and female offspring develop from fertilized eggs. [0003] According to the existing data, the understanding of the fertilized eggs formed by the mating of the male and female mite of A. Jinchuan is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite inserts the sperm bag into the mating hole of the female mite (reference: Liang Lairong, 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 王伯明毕思佳
Owner 山东首伯农生物技术有限公司
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