Manufacturing method of male Amblyseius tsugawai semen slide specimen

Abstract

The invention relates to a manufacturing method of a male Amblyseius tsugawai semen slide specimen. The manufacturing method is characterized in that the manufacturing method is a method for manufacturing a semen slide specimen after acquiring a spermatophore from the jaw body of male Amblyseius tsugawai which is mating, and comprises the following five parts such as acquisition of mating male andfemale Amblyseius tsugawai, shaping of male and female Amblyseius tsugawai, separation of male and female Amblyseius tsugawai, acquisition of the spermatophore, acquisition of semens and slide specimen preparation. The spermatophore of the male Amblyseius tsugawai can be acquired and the semen slide specimen can be manufactured by using an extremely simple and smart method, the acquisition of thespermatophore can provide raw materials for the manufacturing and research of the male Amblyseius tsugawai semen slide specimen, and the manufacturing of the semen slide specimen can provide reference for understanding the shape of Amblyseius tsugawai semens and disclosing the reproduction principle of Amblyseius tsugawai.

Description

technical field [0001] The invention relates to a method for preparing a sperm slide specimen of the male mite of A. tsukawa, and belongs to the field of basic biological research of the a. Background technique [0002] Amblyseius jinchuanensis is a dioecious animal, and only after the mating and fertilization of male and female mites can it lay eggs and breed offspring, and both male and female offspring develop from fertilized eggs. [0003] According to the existing data, the understanding of the fertilized eggs formed by the mating of the male and female mite of A. Jinchuan is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite inserts the sperm bag into the mating hole of the female mite (reference: Liang Lairong, 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertilized egg. Howe...

AI Technical Summary

Problems solved by technology

[0004] However, because the individuals of A. jinsichuan are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data.

Therefore, it is very difficult to obtain spermatozoa, and it is even more difficult to make spermatozoon slide specimens. It is necessary to develop a method to obtain the spermatozoa of A.