Method for obtaining belly-cutting eggs of amblyseius tsugawai

A technology of Amblyseius and adult mite, applied in the field of basic biology research of Amblyseius Jinchuan, can solve the problems of difficult micromanipulation, difficult research, damage, etc., and achieve the effect that is beneficial to the biological control of pests

Inactive Publication Date: 2018-01-16
山东首伯农生物技术有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, due to the small size of A. jinchuan mite, only a few hundred microns, and its fragile body, it is easy to damage it during the operation and cause the outflow of body fluids. It is impossible to carry out any experiments because the body is dry and dry, and it is quite difficult to perform micromanipulation. A single individual obtains a complete tissue sample, so it is very difficult to study from the morphological structure to the cellular level
There are no reports on how to obtain laparotomy eggs, how to take out complete laparotomy eggs, and how to preserve the eggs after they are taken out in the existing materials.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0014] Example A method of obtaining laparotomy eggs of Amblyseius tsukawa

[0015] The specific operation method includes the following 4 parts, all operations are carried out under a stereo microscope:

[0016] (1) Obtaining adult female mites of A. jinsichuan after laparotomy: the eggs of A. jinsichuan were picked from the breeding population of A. jinsichuan and raised separately until they became adults, and then one female and one male were placed in the breeding chamber for pairing , the newly emerged male and female A. tsukawa will mate soon. After the mating is completed, they will continue to provide sufficient prey for the female adults. After 12-15 hours, the abdomen of the female adults will expand and eggs will be obtained. female adult mite;

[0017] (2) Placement of female adults of Amblyseius tsugawan on the glass slide after laparotomy: put a drop of water on the slide, pick the female adults to be dissected and place them in the water on the slide, and use ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to a method for obtaining belly-cutting eggs of amblyseius tsugawai. The method is characterized by manually obtaining unlaid eggs from the body of female adult amblyseius tsugawai in a belly cutting mode, and includes the four steps of obtaining female adult amblyseius tsugawai with the belly to be cut, placing the female adult amblyseius tsugawai on a glass slide, obtainingbelly-cutting eggs of the amblyseius tsugawai and storing the belly-cutting eggs. By means of the method, the belly-cutting eggs of the amblyseius tsugawai can be finely obtained, and successful obtaining of the belly-cutting eggs can provide more basic materials for further research on the amblyseius tsugawai reproduction mechanism, and good understanding of the amblyseius tsugawai reproductionmechanism is beneficial to human-induced changing of the amblyseius tsugawai female-male ratio.

Description

technical field [0001] The invention relates to a method for obtaining laparotomy eggs of A. tsukawa, belonging to the field of basic biology research of A. jinchuan. Background technique [0002] Amblyseius tsukawa is an important natural enemy of small pests. Its reproduction has special characteristics, that is, both male and female offspring develop from fertilized eggs, but the male offspring develops into haploid due to the disappearance of paternal genes after heterochromatinization. This is thought to be determined by the special mode of reproduction of phytoseiid mite, Pseudoarrhenotoky (Hoy, 1985; Nagalkerke & Sabelis 1998; Sabelis et al. 2002). In recent years, in addition to being a natural enemy for biological control, Amblyseius jinchuanensis has also received attention in its biological learning, especially the special reproductive phenomenon of pseudoparenchymal androgenetic reproduction. [0003] Nelson-Rees et al. (1980) observed the karyotype changes duri...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): A01K67/033
Inventor 王伯明毕思佳
Owner 山东首伯农生物技术有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap