Method for obtaining belly-cutting eggs of amblyseius tsugawai

Abstract

The invention relates to a method for obtaining belly-cutting eggs of amblyseius tsugawai. The method is characterized by manually obtaining unlaid eggs from the body of female adult amblyseius tsugawai in a belly cutting mode, and includes the four steps of obtaining female adult amblyseius tsugawai with the belly to be cut, placing the female adult amblyseius tsugawai on a glass slide, obtainingbelly-cutting eggs of the amblyseius tsugawai and storing the belly-cutting eggs. By means of the method, the belly-cutting eggs of the amblyseius tsugawai can be finely obtained, and successful obtaining of the belly-cutting eggs can provide more basic materials for further research on the amblyseius tsugawai reproduction mechanism, and good understanding of the amblyseius tsugawai reproductionmechanism is beneficial to human-induced changing of the amblyseius tsugawai female-male ratio.

Description

technical field [0001] The invention relates to a method for obtaining laparotomy eggs of A. tsukawa, belonging to the field of basic biology research of A. jinchuan. Background technique [0002] Amblyseius tsukawa is an important natural enemy of small pests. Its reproduction has special characteristics, that is, both male and female offspring develop from fertilized eggs, but the male offspring develops into haploid due to the disappearance of paternal genes after heterochromatinization. This is thought to be determined by the special mode of reproduction of phytoseiid mite, Pseudoarrhenotoky (Hoy, 1985; Nagalkerke & Sabelis 1998; Sabelis et al. 2002). In recent years, in addition to being a natural enemy for biological control, Amblyseius jinchuanensis has also received attention in its biological learning, especially the special reproductive phenomenon of pseudoparenchymal androgenetic reproduction. [0003] Nelson-Rees et al. (1980) observed the karyotype changes duri...

AI Technical Summary

Problems solved by technology

[0005] However, due to the small size of A. jinchuan mite, only a few hundred microns, and its fragile body, it is easy to damage it during the operation and cause the outflow of body fluids. It is impossible to carry out any experiments because the body is dry and dry, and it is quite difficult to perform micromanipulation. A single individual obtains a complete tissue sample, so it is very difficult to study from the morphological structure to the cellular level

There are no reports on how to obtain laparotomy eggs, how to take out complete laparotomy eggs, and how to preserve the eggs after they are taken out in the existing materials.