Method for obtaining Amblyseius tsugawai spermatophores and observing Amblyseius tsugawai spermatophores under transmission electron microscope

Abstract

The invention relates to a method for obtaining Amblyseius tsugawai spermatophores and observing the Amblyseius tsugawai spermatophores under a transmission electron microscope. The method is characterized in that spermatophores are obtained from the male gnathosoma of mating Amblyseius tsugawai, and are observed under the transmission electron microscope, and the method includes the steps of obtaining of male and female Amblyseius tsugawais, setting of the male and female Amblyseius tsugawais, separation of the male and female Amblyseius tsugawais, obtaining of the spermatophores, and preparation and observation of a spermatophore transmission electron microscopy sample. The Amblyseius tsugawai male spermatophores can be obtained through a very simple and ingenious technology, and the ultrastructural observation of the internal structure and substances of the outer spermatophores of the Amblyseius tsugawai is carried out by using the transmission electron microscope, so the form and the quantity of sperms in the outer spermatophore and the change (such as heterogenization) or not of the sperms before reaching female Amblyseius tsugawai are clearly known, thereby the determinationof whether the male Amblyseius tsugawai plays a decisive role in the gender determination process or not is benefited.

Description

technical field [0001] The invention relates to the acquisition of the essence of the amblyseius jinchuan and its observation method under a transmission electron microscope, and belongs to the field of basic biological research of the amblyseius jinchuan. Background technique [0002] Amblyseius jinchuanensis is a dioecious animal, and only after the mating and fertilization of male and female mites can it lay eggs and breed offspring, and both male and female offspring develop from fertilized eggs. [0003] According to the existing data, the understanding of the fertilized eggs formed by the mating of the male and female mite of A. Jinchuan is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite inserts the sperm bag into the mating hole of the female mite (reference: Liang Lairong, 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the semina...

AI Technical Summary

Problems solved by technology

[0004] Because the individuals of A. Jinchuan are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites in close contact. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing information

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a transmission electron microscope. It is necessary to develop a method to obtain the spermatozoa of A.