Method for obtaining sperms from predatory male mites and making slide specimens

Abstract

The invention relates to a method for obtaining sperms from predatory male mites and making slide specimens. The method for obtaining the sperms from the predatory male mites and making the slide specimens is characterized by being the method for making the sperm slide specimens after obtaining spermatophore from male mite gnathosoma of the predatory mites in phytoseiidae under mating, comprisingfive parts of acquiring male and female mites under mating, stereotyping the male and female mites, separating the male and female mites, acquiring the spermatophore, acquiring the sperms, and preparing the slide specimens. By adopting the method for obtaining the sperms from the predatory male mites and making the slide specimens disclosed by the invention, the spermatophore of the predatory malemites can be obtained by using a very simple and ingenious method, and the sperm slide specimens can be made, the spermatophore is obtained, thereby being capable of providing raw materials for making research on the sperm slide specimens of the predatory mites, and the sperm slide specimens can be made, thereby being capable of providing a reference for understanding the morphology of the predatory mite sperms and revealing reproduction principles of the predatory mites.

Description

technical field [0001] The invention relates to a method for obtaining the sperm of predatory male mite and making a glass slide specimen, which belongs to the field of non-biological basic research. Background technique [0002] Predatory mites of Phytoseiidae are dioecious animals, and only after the mating and fertilization of male and female mites can they lay eggs and breed offspring, and the offspring, regardless of sex, develop from fertilized eggs. [0003] According to the existing data, the understanding of the mating of male and female mites of Phytoseiidae to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (references: Liang Lairong, 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertili...

AI Technical Summary

Problems solved by technology

[0004] However, due to the fact that the individual predatory mites of Phytoseiidae are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only 1 hour The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain spermatozoa, and it is even more difficult to make spermatozoon slide specimens. It is necessary to develop a method to obtain and make sperm slide specimens of Phytoseiidae predator mite