Method for obtaining Neoseiulus californicus spermatophores and observing Neoseiulus californicus spermatophores under transmission electron microscope

Abstract

The invention relates to a method for obtaining Neoseiulus californicus spermatophores and observing the Neoseiulus californicus spermatophores under a transmission electron microscope. The method ischaracterized in that spermatophores are obtained from the male gnathosoma of mating Neoseiulus californicus, and are observed under the transmission electron microscope, and the method includes the steps of obtaining of male and female Neoseiulus californicuss, setting of the male and female Neoseiulus californicuss, separation of the male and female Neoseiulus californicuss, obtaining of the spermatophores, and preparation and observation of a spermatophore transmission electron microscopy sample. The Neoseiulus californicus male spermatophores can be obtained through a very simple and ingenious technology, and the ultrastructural observation of the internal structure and substances of the outer spermatophores of the Neoseiulus californicus is carried out by using the transmission electron microscope, so the form and the quantity of sperms in the outer spermatophore and the change (such as heterogenization) or not of the sperms before reaching female Neoseiulus californicus are clearly known, thereby the determination of whether the male Neoseiulus californicus plays a decisive role in the gender determination process or not is benefited.

Description

technical field [0001] The invention relates to a method for obtaining and observing the spermatozoa of Neoseius californica under a transmission electron microscope, and belongs to the field of basic biological research of Neoseius californica. Background technique [0002] Neoseius californica is a dioecious animal. It must undergo mating and fertilization between male and female mites before laying eggs and breeding offspring. Both male and female offspring develop from fertilized eggs. [0003] The existing information on the mating of male and female mite of Neoseius californica to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg t...

AI Technical Summary

Problems solved by technology

[0004] Because the individuals of Neoseius california are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data.

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a transmission electron microscope. It is necessary to develop a method to obtain the spermatozoa of Neoseius californica and observe the spermatozoa with a transmission electron microscope.