Transmission electron microscope observation method for male spermatophore of phyoseiulus persimilis

Abstract

The invention relates to a transmission electron microscope observation method for male spermatophore of phyoseiulus persimilis. The method is capable of receiving the spermatophore from gnathosoma of the mated phyoseiulus persimilis and observing the spermatophore under a transmission electron microscope, comprises five steps of acquisition of female and male acarid, determination of the female and male acarid, separation of the female and male acarid, acquisition of the spermatophore, and preparation and observation of a spermatophore transmission electron microscope sample. The male spermatophore of the phyoseiulus persimilis can be obtained by using the simple and smart method, and the transmission electron microscope is used for executing the ultramicro observation to the internal structure and substances of the external spermatophore of the phyoseiulus persimilis. Through the observation, the forms and number of sperms in the external spermatophore are further determined and the change (such as: heterogenization) whether to be generated before the sperms reach the internal body of the female acarid and so on. The method is good for determining whether the male acarid of the phyoseiulus persimilis has the crucial effect in the sex determination process.

Description

technical field [0001] The invention relates to a transmission electron microscope observation method of the male phytoseiid mite semen, and belongs to the field of basic biological research of the phytoseiid mite. Background technique [0002] Phytoseius militaris is a dioecious animal, and the male and female mites must be mated and fertilized before laying eggs and breeding offspring, and both male and female offspring develop from fertilized eggs. [0003] Existing information on the formation of fertilized eggs by the mating of male and female mites of Phytoseius militaris is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertilized egg. Howe...

AI Technical Summary

Problems solved by technology

[0004] Because the individuals of Phytoseius militaris are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data.

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a transmission electron microscope. It is necessary to develop a method to obtain the spermatozoa of Phytoseiillus militaris and observe the spermatozoa with a transmission electron microscope.