Non-directional mating litopenaeus vannamei full-sib family batch preparation method

Abstract

The invention discloses a non-directional mating litopenaeus vannamei full-sib family batch preparation method. According to defects of an existing litopenaeus vannamei family preparation technology, the method in which non-directional mating is used, litopenaeus vannamei beard tissue is sheared to extract DNA, and remaining sperms in spermatophores of female litopenaeus vannamei obtained after oviposition serve as a male parent source basis is designed, a male parent individual and a female parent individual are determined by means of an existing mature microsatellite parentage identification technology, and a full-sib family is prepared. The method which combines high non-directional random mating efficiency and high microsatellite parentage identification accuracy is used, the defects of the existing litopenaeus vannamei family preparation technology are made up for, and the method is beneficial to litopenaeus vannamei genetic breeding.

Description

Technical field [0001] The invention belongs to the field of genetic breeding, and specifically relates to a batch preparation method of a non-directional mating Litopenaeus vannamei all-sib family. Background technique [0002] Litopenaeus vannamei (Litopenaeus vannamei, also known as Penaeus vannamei) is the pillar industry of my country's aquaculture, and it occupies a large proportion of China's fishery economy. However, the high-quality broodstock of Litopenaeus vannamei has long been dependent on imports, which has severely restricted the stable and rapid development of my country's shrimp industry. Therefore, strengthening the research on genetic breeding of Litopenaeus vannamei will contribute to the rapid and healthy development of my country's prawn industry, which is of great significance. [0003] The preparation of all-sibling families is the most important link in the field of genetic breeding. All offspring individuals of a full-sib family come from the same parent...

AI Technical Summary

Problems solved by technology

[0004] However, there are still some technical defects in the existing preparation method of Litopenaeus vannamei full-sib family

Existing non-directional mating method: generally all adopt marker to carry out labeling to broodstock, as putting on the identifiable marker on a certain part of broodstock, just write down marker when broodstock mates, so that determine parent, but this The first method is sometimes unable to determine the male parent, the female shrimp will mate with multiple male shrimp, and it is not sure which male shrimp successfully penetrated into the seminal vesicle of the female shrimp

The existing method of directional mating: generally put one or more male shrimps and one female shrimp in the same water body to match, because it cannot guarantee whether the specific combination will successfully mate, this method usually needs to prepare more The number of full-sibling families can be obtained only by combination, and the chasing space of the water body is limited, so there are often unfavorable factors such as sperm pods being crooked or falling off

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