Non-directional mating litopenaeus vannamei full-sib family batch preparation method

A full-sib family and Fannabin technology, applied in the field of genetics and breeding, can solve problems such as deflected sperm pods, shedding of sperm pods, and limited space for water bodies to chase

Active Publication Date: 2017-05-10
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
View PDF6 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, there are still some technical defects in the existing preparation method of Litopenaeus vannamei full-sib family
Existing non-directional mating method: generally all adopt marker to carry out labeling to broodstock, as putting on the identifiable marker on a certain part of broodstock, just write down marker when broodstock mates, so that determine parent, but this The first method is sometimes unable to determine the male parent, the female shrimp will mate with multiple male shrimp, and it is not sure which male shri...

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Non-directional mating litopenaeus vannamei full-sib family batch preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] 1. Parent selection: Generally, healthy individuals with a weight of 20g or more (male shrimps 20g or more, female shrimps 30g or more) are selected as backup parents, and the parents are identified one by one. The first pair of swimming feet with split structure or spermatopod is the male shrimp , Individuals with no bifurcated structure, no seed pods or obvious ovaries are female shrimp, and the male and female are reared separately.

[0025] 2. Parental treatment: Use red-hot tweezers or scissors to wring off or cut off one side of the eye stalk of the female shrimp. The male prawns do not cut the eye stalk; cut all female and male individual beards close to the root. The beards of each individual were stored separately in 95% alcohol at -20°C, and the test tube numbers were managed. The females were raised in the female ponds, and the males were raised in the male ponds.

[0026] 3. Broodstock feeding: select live fresh bait and feed it once every 6-8 hours. The amount o...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention discloses a non-directional mating litopenaeus vannamei full-sib family batch preparation method. According to defects of an existing litopenaeus vannamei family preparation technology, the method in which non-directional mating is used, litopenaeus vannamei beard tissue is sheared to extract DNA, and remaining sperms in spermatophores of female litopenaeus vannamei obtained after oviposition serve as a male parent source basis is designed, a male parent individual and a female parent individual are determined by means of an existing mature microsatellite parentage identification technology, and a full-sib family is prepared. The method which combines high non-directional random mating efficiency and high microsatellite parentage identification accuracy is used, the defects of the existing litopenaeus vannamei family preparation technology are made up for, and the method is beneficial to litopenaeus vannamei genetic breeding.

Description

Technical field [0001] The invention belongs to the field of genetic breeding, and specifically relates to a batch preparation method of a non-directional mating Litopenaeus vannamei all-sib family. Background technique [0002] Litopenaeus vannamei (Litopenaeus vannamei, also known as Penaeus vannamei) is the pillar industry of my country's aquaculture, and it occupies a large proportion of China's fishery economy. However, the high-quality broodstock of Litopenaeus vannamei has long been dependent on imports, which has severely restricted the stable and rapid development of my country's shrimp industry. Therefore, strengthening the research on genetic breeding of Litopenaeus vannamei will contribute to the rapid and healthy development of my country's prawn industry, which is of great significance. [0003] The preparation of all-sibling families is the most important link in the field of genetic breeding. All offspring individuals of a full-sib family come from the same parent...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): A01K61/59
CPCY02A40/81
Inventor 黄文陈廷任春华江晓王艳红胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap