Method for observing spermatophore of predatory mite under scanning electron microscope

Abstract

The invention relates to a method for observing a spermatophore of a predatory mite under a scanning electron microscope. The method is characterized by steps of acquiring a predatory mite male with an easy-to-see spermatophore at first and then preparing and observing the spermatophore under the scanning electron microscope, and comprises five parts of acquisition of predatory mite male and female in mating, sizing of predatory mite male and female, separation of predatory mite male and female, storage of predatory mite male, and spermatophore scanning electron microscope specimen preparationand observation. According to the invention, the easy and ingenious method is utilized to acquire the spermatophore of the predatory mite male, and realize the purpose of observing the tiny externalstructure of the spermatophore under the scanning electron microscope; the acquisition of the spermatophore provides a raw material for observing the structure of the spermatophore under the scanningelectron microscope, and a result of the scanning electron microscope facilitates the foundation of research on other respects of the spermatophore and gives enlightenment.

Description

technical field [0001] The invention relates to a method for observing spermatozoa of predatory mite under a scanning electron microscope, and belongs to the field of basic biological research of predatory mite. Background technique [0002] Predatory mites of Phytoseiidae are dioecious animals, and only after the mating and fertilization of male and female mites can they lay eggs and breed offspring, and the offspring, regardless of sex, develop from fertilized eggs. [0003] The existing information on the formation of fertilized eggs by the mating of male and female mite predators is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (reference: Liang Lairong, 1979 , plant protection, how mites live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertilized egg....

AI Technical Summary

Problems solved by technology

[0004] Because the individual predator mite is very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. The size is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites in close contact. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a scanning electron microscope. It is necessary to develop a method to obtain the spermatozoa of predatory mite and observe the spermatozoa with a scanning electron microscope.