Method for extracting macrobrachium spermatophore through pulse electric shock method and macrobrachium intraspecific crossbreeding method

Abstract

The invention discloses a method for extracting macrobrachium spermatophore through a pulse electric shock method and a macrobrachium intraspecific crossbreeding method. The methods comprise the steps that two pulse electrode tips are put on the portions close to the rear sides of two fifth paraeiopod bases, a connecting lead between the two electrode tips is parallel to the junction loop line of the cephalothorax and the abdomen of male macrobrachium, the electrode tips are basically perpendicular to the abdomen surface of the male macrobrachium, a pulse power switch is gently clicked once or twice, and the male macrobrachium is simulated through pulse electricity; a person pays close attention to spermatophore discharging holes formed in the inner sides of the fifth paraeiopod bases, takes down the spermatophore as soon as finding the spermatophore with ophthalmological tweezers and rapidly stick the spermatophore to the portions between the fourth paraeiopod bases and the fifth paraeiopod bases of congener female macrobrachium which just finishes reproductive uncoating, and the female macrobrachium conducts oviposition and incubation by itself. According to the method for extracting the macrobrachium spermatophore through the pulse electric shock method and the macrobrachium intraspecific crossbreeding method, the spermatophore can be extracted from the male macrobrachium rapidly, effectively and safely, the pulse electricity is adopted to extract the spermatophore, the male macrobrachium is not damaged, and compared with stimulation through an ordinary alternating current, the extracting success rate, the transplanting success rate and the fertilization rate are all obviously increased.

Description

technical field

[0001] The invention belongs to the technical field of aquatic animal reproduction and breeding, and relates to the key steps of artificial insemination in macrobrachium crossbreeding, in particular to the technology of electric pulse stimulation to extract the sperm pods of macrobrach male shrimp. Background technique

[0002] Macrobrachium is an important economic shrimp in my country, especially the freshwater prawn and Macrobrachium rosenbergii in Macrobrachium, which are important freshwater cultured shrimp in my country. According to the "China Fishery Yearbook", in 2013, the annual output of freshwater shrimp and Macrobrachium rosenbergii in my country was 251,000 tons and 117,000 tons respectively. The number of farming provinces reached 21 and 16 respectively. However, the phenomenon of serious species degeneration has occurred, the individuals have become smaller, the number of diseases has increased, and the breeding benefits have declined year by y...

Examples

Embodiment 1

[0020] Green shrimp sperm pod extraction and transplantation: using the electrode head designed by the present invention, using 30V pulse voltage, pulse width 35μS, sperm pod extraction rate is 96%, the success rate of sperm pod transplantation to female green shrimp body is 87%, and the fertilization rate is 92%. %.

Embodiment 2

[0022] Extraction and transplantation of sperm pods of Macrobrachium hainanensis: using the electrode head designed by the present invention, using 31V pulse voltage, pulse width 40μS, sperm pod extraction rate is 94%, the success rate of sperm pod transplantation to female Macrobrachium hainanensis is 85%, fertilization The rate is 90.5%.

Embodiment 3

[0024] Macrobrachium rosenbergii sperm pod extraction and transplantation: using the electrode head designed in the present invention, using 34V pulse voltage, pulse width 60μS, sperm pod extraction rate 93%, sperm pod transplantation success rate on female Macrobrachium rosenbergii body 83%, fertilization The rate is 89%.

technical field

[0001] The invention belongs to the technical field of aquatic animal reproduction and breeding, and relates to the key steps of artificial insemination in macrobrachium crossbreeding, in particular to the technology of electric pulse stimulation to extract the sperm pods of macrobrach male shrimp. Background technique

[0002] Macrobrachium is an important economic shrimp in my country, especially the freshwater prawn and Macrobrachium rosenbergii in Macrobrachium, which are important freshwater cultured shrimp in my country. According to the "China Fishery Yearbook", in 2013, the annual output of freshwater shrimp and Macrobrachium rosenbergii in my country was 251,000 tons and 117,000 tons respectively. The number of farming provinces reached 21 and 16 respectively. However, the phenomenon of serious species degeneration has occurred, the individuals have become smaller, the number of diseases has increased, and the breeding benefits have declined year by y...

Examples

Embodiment 1

[0020] Green shrimp sperm pod extraction and transplantation: using the electrode head designed by the present invention, using 30V pulse voltage, pulse width 35μS, sperm pod extraction rate is 96%, the success rate of sperm pod transplantation to female green shrimp body is 87%, and the fertilization rate is 92%. %.

Embodiment 2

[0022] Extraction and transplantation of sperm pods of Macrobrachium hainanensis: using the electrode head designed by the present invention, using 31V pulse voltage, pulse width 40μS, sperm pod extraction rate is 94%, the success rate of sperm pod transplantation to female Macrobrachium hainanensis is 85%, fertilization The rate is 90.5%.

Embodiment 3

[0024] Macrobrachium rosenbergii sperm pod extraction and transplantation: using the electrode head designed in the present invention, using 34V pulse voltage, pulse width 60μS, sperm pod extraction rate 93%, sperm pod transplantation success rate on female Macrobrachium rosenbergii body 83%, fertilization The rate is 89%.

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