Method for observing predatory mite spermatophore under transmission electron microscope

Abstract

The invention relates to a method for observing predatory mite spermatophore under a transmission electron microscope. The method is characterized by a method for acquiring spermatophore from male phytoseiidae predatory mite capitulum in the mating process and then observing the spermatophore under the transmission electron microscope. The method comprises the following five steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; acquiring spermatophore; preparing and observing a spermatophore transmission electron microscope sample. The invention utilizes a simple and smart method to acquire the male phytoseiidae predatory mite spermatophore; the transmission electron microscope is utilized to ultra-micro observe the inner structure and matter of the spermatophore of the predatory mite; through observation, we can further know the form and quantity of the sperms in the spermatophore and if the sperms have changed (such as, heterogenization) before entering into the female mite body, so that people can confirm if the male phytoseiidae predatory mite plays a decisive role in a sex determination process.

Description

technical field [0001] The invention relates to a method for observing the semen of predatory mite under a transmission electron microscope, and belongs to the field of basic biological research of predatory mite of Phytoseiidae. Background technique [0002] Predatory mites of Phytoseiidae are dioecious animals, and only after the mating and fertilization of male and female mites can they lay eggs and breed offspring, and the offspring, regardless of sex, develop from fertilized eggs. [0003] According to the existing data, the understanding of the mating of male and female mites of Phytoseiidae to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (references: Liang Lairong, 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined ...

AI Technical Summary

Problems solved by technology

[0004] Because the individual predator mite is very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. The size is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites in close contact. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a transmission electron microscope. It is necessary to develop a method to obtain the spermatozoa of predatory mite and observe the spermatozoa with a transmission electron microscope.