Litopenaeus vannamei male parent semi-directional mating full-sib family construction and parent determination method

Abstract

The invention relates to a litopenaeus vannamei male parent semi-directional mating full-sib family construction and parent determination method. The method sequentially comprises the following steps of female parent management, breeding and screening of candidate mating male parents, male parent semi-directional mating, parent determination, fertilized egg treatment, larva breeding and shrimp seed cultivation. According to the method, in combination with the breeding habits and characteristics of litopenaeus vannamei, one male shrimp with milky spermatophore, full sperm mass filling and high chasing ability is screened out from the candidate mating male parents, and the male shrimp and five female shrimps with full ovaries are subjected to semi-directional mating, so that the mating success rate is increased. According to the method, the full-sib family of the litopenaeus vannamei can be quickly and efficiently constructed, a large number of full-sib family filial generations can be obtained, and parents of the full-sib family can be simply and quickly determined, so that genetic and character characteristics of the parents of the full-sib family can be completely and accurately obtained; and therefore, an important technical support is provided for molecular marker-assisted breeding of the litopenaeus vannamei.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a method for constructing a full-sib family and determining a parent of Litopenaeus vannamei for semi-directed mating of male parents. Background technique [0002] Litopenaeus vannamei (Litopenaeus vannamei, also known as vannamei) aquaculture is one of the pillar industries of aquaculture in my country. However, for high-quality broodstock of Litopenaeus vannamei, my country has long relied on imports, and domestic breeding is still dominated by traditional selection. Strengthening the research on molecular genetic breeding technology of Litopenaeus vannamei will help improve the unfavorable situation of my country's Litopenaeus vannamei industry and promote the healthy development of the shrimp farming industry. [0003] At present, in the existing full-sib family construction technology, the male parent and the female parent can be directly determined by...

AI Technical Summary

Problems solved by technology

[0003] At present, in the existing full-sib family construction technology, the male parent and the female parent can be directly determined by taking sperm, eggs and mixing them directly, but this method needs to kill broodstock, and the fertilization rate of this method is low. It is impossible to obtain a large number of offspring; however, the method of obtaining male shrimp DNA by using the sperm pod tissue remaining in the body of the female shrimp, and then confirming the male shrimp through the paternity identification technology can obtain more offspring, but the follow-up parentage identification procedure has many steps and is relatively cumbersome