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Litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method

A technology of full-sib family and determination method, which is applied in the field of semi-directional mating full-sib family construction and parental determination of Litopenaeus vannamei, which can solve the problems of tediousness, inability to obtain a large number of offspring, and low fertilization rate

Active Publication Date: 2018-01-16
SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the existing full-sib family construction technology can directly determine the father and mother through the method of extracting sperm and eggs and directly mixing them, but the parent shrimp needs to be killed, and the fertilization rate of this method is low, and a large number of offspring cannot be obtained; the use of female shrimp The sperm pod tissue remaining in the body represents the DNA of the male shrimp, and then the method of determining the male shrimp through the paternity test technology can obtain more offspring, but the subsequent parentage test procedure has many steps and is cumbersome

Method used

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  • Litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method

Examples

Experimental program
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Effect test

Embodiment 1

[0021] (1) Parental management: the eye stalk of the female shrimp is cut off, and the eye stalk of the male shrimp is not cut. The female shrimp and the male shrimp are raised in the female shrimp pond and the male shrimp pond respectively, and the live caterpillar and red worm bait are fed, and the female shrimp pond is maintained. The living bait in the male shrimp pond is uninterrupted, the water depth of the female shrimp pond is 40cm, and the water depth of the male shrimp pond is 70cm; after 7 days, the female shrimp's gonads are full and ready to start mating;

[0022] (2) Semi-directed mating: at 7:00 in the morning, select 40 male shrimps with milky white sperm pods and full sperm mass and put them into another pool as a mating pool. The depth of water in the mating pool is 70cm, and the area is large 10m 2 ; Select a female shrimp with full gonads and put it into the mating tank to carry out semi-directional mating with a large number of male shrimp; at 17:00, if th...

Embodiment 2

[0027] (1) Parental management: the eye stalk of the female shrimp is cut off, and the eye stalk of the male shrimp is not cut. The female shrimp and the male shrimp are raised in the female shrimp pond and the male shrimp pond respectively, and the live caterpillar and red worm bait are fed, and the female shrimp pond is maintained. The living bait in the male shrimp pond is uninterrupted, the water depth of the female shrimp pond is 30cm, and the water depth of the male shrimp pond is 65cm; after 7 days, the female shrimp's gonads are full and ready to start mating;

[0028] (2) Semi-directed mating: At 6:00 in the morning, select 20 male shrimps with milky white sperm pods and full sperm mass and put them into another pool as a mating pool. The depth of water in the mating pool is 65cm, the area is large 5m 2 ; Select a female shrimp with full gonads and put it into the mating tank for semi-directional mating with a large number of male shrimp; at 16:00, if the seminal vesi...

Embodiment 3

[0033] (1) Parental management: the eye stalk of the female shrimp is cut off, and the eye stalk of the male shrimp is not cut. The female shrimp and the male shrimp are raised in the female shrimp pond and the male shrimp pond respectively, and the live caterpillar and red worm bait are fed, and the female shrimp pond is maintained. The live bait in the male shrimp pond is uninterrupted, the water depth in the female shrimp pond is 45cm, and the water body depth in the male shrimp pond is 100cm; after 7 days, the female shrimp's gonads are full and ready to start mating;

[0034] (2) Semi-directed mating: At 9:00 in the morning, select 60 male shrimps whose sperm pods are milky white and full of sperm mass and put them into another pool as a mating pool. The depth of water in the mating pool is 100 cm, and the size 15m 2 ; Select a female shrimp with full gonads and put it into the mating tank for semi-directional mating with a large number of male shrimp; at 19:00, if the se...

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Abstract

The invention discloses a litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method. By performing semi-directional mating between one female shrimp and many male shrimps, the mating success rate is ensured, and then by examining whether the spermatophore of the male shrimps is empty or not one to one, a male parent is determined. According to the litopenaeus vannamei semi-directional mating full sibs family construction and parent determination method, a litopenaeus vannamei full sibs family can be efficiently constructed, and a large quantityof the full sibs family filial generations are obtained; by the adoption of the method, the parent of the full sibs family can be conveniently and fast determined so that heredity and trait characteristics of the full sibs family parent can be completely obtained. The method provides important technological support for litopenaeus vannamei molecular marker assistant breeding.

Description

technical field [0001] The invention belongs to the technical field of genetic breeding, and in particular relates to a method for constructing a semi-directional mating full-sib family of Litopenaeus vannamei and determining parents. Background technique: [0002] Litopenaeus vannamei (Litopenaeus vannamei, also known as Penaeus vannamei) aquaculture is one of the pillar industries of aquaculture in my country. However, my country's high-quality broodstock has long relied on imports, and domestic breeding is still dominated by traditional selection. Strengthening the research on molecular genetic breeding technology of Litopenaeus vannamei will help improve the unfavorable situation of my country's Litopenaeus vannamei industry and promote the healthy development of the shrimp farming industry. [0003] Full-sib families are indispensable experimental materials in molecular genetic breeding. Through the comparison of trait differences between different individuals of full-s...

Claims

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Application Information

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IPC IPC(8): A01K61/59
CPCY02A40/81
Inventor 黄文任春华程楚杭陈廷江晓王艳红胡超群
Owner SOUTH CHINA SEA INST OF OCEANOLOGY - CHINESE ACAD OF SCI
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