Application of TGL gene to prevention and control of sitotroga cerealella

A wheat moth and gene technology, applied in application, insecticide, animal repellent, etc., can solve the problems of less research on insect TGL gene function and unclear mechanism.

Active Publication Date: 2020-02-14
HUAZHONG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few studies on the function of insect TGL genes, and the specific mechanism is still unclear.

Method used

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  • Application of TGL gene to prevention and control of sitotroga cerealella
  • Application of TGL gene to prevention and control of sitotroga cerealella
  • Application of TGL gene to prevention and control of sitotroga cerealella

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Cloning of TGL gene and its expression pattern in space and time

[0033] 1. Materials and methods

[0034] 1.1 Insects to be tested

[0035] The wheat moths used in the experiment are the wheat moths that were raised for multiple generations under the same conditions in the same room as the grain storage of farmers. They were raised in round-bottomed transparent glass bottles (10 cm in height and 4 cm in radius). Clean wheat grains about 2 cm thick were placed in the bottles and placed at a temperature of 28 ±1°C, relative humidity 75±5%, photoperiod L:D=14:10 artificial climate chamber. Select wheat grains containing pupae and raise them separately in finger tubes, check them daily, and take the virgin moths that have just emerged, and distinguish between male and female for later use.

[0036] 1.2 Instruments and main reagents

[0037] 1.2.1 Instruments and equipment

[0038] Neofuge 13R desktop high-speed refrigerated centrifuge (Shanghai Lishen Scientific Instr...

Embodiment 2

[0089] Functional Study of TGL Gene

[0090] 1 Materials and methods

[0091] 1.1 Insects to be tested

[0092] With embodiment 1, get the virgin male moth that just emerges, for subsequent use.

[0093] 1.2 Instruments and main reagents

[0094] 1.2.1 Instruments and equipment

[0095]SZM series stereo microscope (Sunyu Instrument Co., Ltd.); Neofuge 13R desktop high-speed refrigerated centrifuge (Shanghai Lishen Scientific Instrument Co., Ltd.); gel imager Gel Doc XR Plus (Bio-Rad, USA); horizontal nucleic acid electrophoresis Instrument Power Station AE-8450; Nucleic acid and protein analyzer Biophotometer (Eppendorf, Germany); Real-time fluorescent quantitative PCR instrument CFX 96 (Bio-Rad, USA); Nanoliter2010 microinjection instrument (WPI, USA).

[0096] 1.2.2 Main reagents

[0097] Gel Recovery Kit (Axygen); Plasmid Miniprep Kit (Axygen); Competent Cells: DH5α Chemically Competent Cell (Shanghai Yisheng Biological Co., Ltd.); Vector: PMD-19T (TaKaRa); SYBR green ...

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PUM

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Abstract

The invention provides application of a TGL gene to prevention and control of sitotroga cerealella, and relates to the technical field of insect prevention and control. When the expression of the TGLgene is suppressed, the decomposition rate of triacylglycerol is reduced, so that sufficient mating contents are not synthesized in male insect bodies, and therefore, no more contents can be transferred to female insects in the mating process, normal spermatophores cannot be formed inside copulatory bursa, and eventually mature eggs cannot be successfully fertilized.

Description

technical field [0001] The invention belongs to the technical field of agricultural pest prevention and control, and specifically relates to the application of TGL gene in the prevention and control of wheat moths. Background technique [0002] The wheat moth S. cerealella Olivier is a worldwide grain pest and one of the three major stored grain pests in my country. The annual damage of the wheat moth leads to a mass loss rate of about 65% of wheat and rice (Li Guangtao et al., 2008; Wang Dianxuan et al., 2017). At present, the control methods of wheat moth mainly rely on chemical control, but due to the enhancement of insecticide resistance of wheat moth and the characteristics of in-grain damage of larvae, chemical control methods are difficult to achieve ideal results. Therefore, the control of adult wheat moth is to reduce the pest. the most effective way of doing harm. [0003] Triacylglycerol plays an important role in cell formation and energy metabolism, including p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N57/16A01P7/04
CPCA01N57/16
Inventor 杨凤连吴梦雅闫文晗吴刚张素素姚雨晨
Owner HUAZHONG AGRI UNIV
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