Method for acquiring and observing neoseiulus californicus spermatophores under scanning electron microscope

A scanning electron microscope, the technology of Microseiid mites, which is applied in the field of basic research on the biology of the new seiid mites in California, can solve the problems of difficult, difficult to see, and difficult to observe the spermatozoa by scanning electron microscope.

Inactive Publication Date: 2017-12-26
合肥天敌之家生物技术有限公司
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Problems solved by technology

[0004] Because the individuals of Neoseius california are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which...

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Embodiment

[0013] Example A method of obtaining and observing the spermatozoa of Neoseius californica under a scanning electron microscope

[0014] This method is a method of artificially obtaining the spermatozoa from the mating mandibles of Neoseius californica males, and then observing the spermatozoa under a scanning electron microscope, including the acquisition of the male and female mites during mating, the typing of the male and female mites, and the identification of the male and female mites. Separation, acquisition of spermatozoa, scanning electron microscope observation 5 parts, the specific operation is:

[0015] (1) Acquisition of male and female mites during mating: Under the microscope, pick out the eggs of Neoseius californica from the breeding population of Neoseius californica and rear them separately until they become adults, then pair the male and female mites, and pair the male and female mites for about 10 minutes It can be mated, so as to obtain the male and femal...

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Abstract

The invention relates to a method for acquiring and observing neoseiulus californicus spermatophores under a scanning electron microscope. The method is characterized in that male neoseiulus californicus easily seen spermatophores are firstly acquired, and the spermatophores are prepared and observed under the scanning electron microscope. The method includes the steps: acquisition of male and female neoseiulus californicus in mating; shaping of the male and female neoseiulus californicus; separation of the male and female neoseiulus californicus; storage of the male neoseiulus californicus; preparation and observation of spermatophore scanning electron microscope specimens. The male neoseiulus californicus spermatophores can be acquired by a quite simple, easy and ingenious method, fine external structures of the spermatophores can be observed under the scanning electron microscope, acquisition of the spermatophores can provide raw materials for observation of the spermatophore structure under the scanning electron microscope, and results of the scanning electron microscope lay a foundation and provides an enlightenment for research of other directions of the spermatophores.

Description

technical field [0001] The invention relates to a method for obtaining and observing the spermatozoa of Neoseius californica under a scanning electron microscope, and belongs to the field of basic biological research of Neoseius californica. Background technique [0002] Neoseius californica is a dioecious animal. It must undergo mating and fertilization between male and female mites before laying eggs and breeding offspring. Both male and female offspring develop from fertilized eggs. [0003] The existing information on the mating of male and female mite of Neoseius californica to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to fo...

Claims

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Application Information

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IPC IPC(8): G01Q30/20G01Q30/02
CPCG01Q30/20G01Q30/02
Inventor 王伯明杨静逸
Owner 合肥天敌之家生物技术有限公司
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