Method for acquiring and observing neoseiulus californicus spermatophores under scanning electron microscope

Abstract

The invention relates to a method for acquiring and observing neoseiulus californicus spermatophores under a scanning electron microscope. The method is characterized in that male neoseiulus californicus easily seen spermatophores are firstly acquired, and the spermatophores are prepared and observed under the scanning electron microscope. The method includes the steps: acquisition of male and female neoseiulus californicus in mating; shaping of the male and female neoseiulus californicus; separation of the male and female neoseiulus californicus; storage of the male neoseiulus californicus; preparation and observation of spermatophore scanning electron microscope specimens. The male neoseiulus californicus spermatophores can be acquired by a quite simple, easy and ingenious method, fine external structures of the spermatophores can be observed under the scanning electron microscope, acquisition of the spermatophores can provide raw materials for observation of the spermatophore structure under the scanning electron microscope, and results of the scanning electron microscope lay a foundation and provides an enlightenment for research of other directions of the spermatophores.

Description

technical field [0001] The invention relates to a method for obtaining and observing the spermatozoa of Neoseius californica under a scanning electron microscope, and belongs to the field of basic biological research of Neoseius californica. Background technique [0002] Neoseius californica is a dioecious animal. It must undergo mating and fertilization between male and female mites before laying eggs and breeding offspring. Both male and female offspring develop from fertilized eggs. [0003] The existing information on the mating of male and female mite of Neoseius californica to form fertilized eggs is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to fo...

AI Technical Summary

Problems solved by technology

[0004] Because the individuals of Neoseius california are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, only about 1 hour. The size of the spermatozoa is only a dozen microns and it is hidden between the ventral surfaces of the male and female mites, which are in close contact with each other. It is difficult to see intuitively, and there is no way to obtain the spermatozoa in the existing data.

Therefore, it is very difficult to obtain the spermatozoa, and it is even more difficult to observe the spermatozoa with a scanning electron microscope. It is necessary to develop a method to obtain the spermatozoa of Neoseius californica and observe the spermatozoa with a scanning electron microscope.