Method for acquiring phyoseiulus persimilis spermatophore

A technology of phytoseiid militaris and spermatozoa, applied in cell dissociation methods, biochemical equipment and methods, preservation of human or animal bodies, etc., can solve problems such as no way to obtain spermatozoa, difficulty in seeing, etc.

Inactive Publication Date: 2017-12-15
SAFE BEST & NECESSARY BEIJING BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] However, because the individuals of Phytoseioid mites are very small, only a few hundred microns, the spermatozoa only appear when the male and female mites mate. During the mating process, the male and female mites are very closely stacked together, and the mating time is very short, on

Method used

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Examples

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Effect test

Embodiment

[0012] Example The method for obtaining the semen of Phytoseiid militarum Chile

[0013] This method is a method of artificially obtaining and picking out the spermatozoa from the jaws of the male phytoseioid mites that are mating, including the acquisition of male and female mites during mating, the typing of male and female mites, the separation of male and female mites, and the acquisition of spermatocysts. In the 4th part of preservation, all operations are carried out under a microscope except for the determination of the male and female mites during mating. The specific operations are:

[0014] (1) Acquisition of male and female mites during mating: From the rearing population of Phytoseius militaris, pick out the eggs of Phytoseiillus militaris and rear them separately until they become adults, then pair the male and female mites. After pairing for about 10 minutes, the male and female mites can mate. Thereby obtaining mating male and female mites;

[0015] (2) The ste...

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Abstract

The invention relates to a method for acquiring phyoseiulus persimilis spermatophore. The method is characterized by being the method for manually acquiring and picking spermatophore from male phyoseiulus persimilis capitulum in the mating process. The method comprises the following four steps: acquiring male and female mites in the mating process; shaping male and female mites; separating male and female mites; and acquiring and storing spermatophore. All the operations, except for the shaping of the male and female mites in the mating process, are performed under the microscope. The invention utilizes a simple and smart method to acquire the phyoseiulus persimilis spermatophore; the acquisition for the spermatophore is capable of supplying material source for the research on the phyoseiulus persimilis sperm; the invention also can supply reference for the acquisition for the other colony predatory mite spermatophore.

Description

technical field [0001] The invention relates to a method for obtaining phytoseiid militaris semen capsules, and belongs to the field of basic biological research of phytoseiid militaris militaris. Background technique [0002] Phytoseius militaris is a dioecious animal, and the male and female mites must be mated and fertilized before laying eggs and breeding offspring, and both male and female offspring develop from fertilized eggs. [0003] Existing information on the formation of fertilized eggs by the mating of male and female mites of Phytoseius militaris is that the sperm of the male mite is stored in the sperm bag before entering the body of the female mite, and the female mite stuffs the sperm bag into the mating hole of the female mite (Reference: Liang Lairong , 1979, Plant Protection, How Mites Live), the sperm is slowly released from the seminal vesicle, transferred to the seminal vesicle and combined with the egg to form a fertilized egg. However, there is no r...

Claims

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Application Information

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IPC IPC(8): C12N5/07A01K67/033A01N1/02
CPCC12N5/0601A01K67/033A01K2227/70A01N1/021C12N2509/10
Inventor 姜晓环王伯明
Owner SAFE BEST & NECESSARY BEIJING BIOTECH CO LTD
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