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Biological engineering method of squids

A squid and artificial technology, applied in the field of squid artificial breeding, can solve the problems of affecting market value, individual reduction, poor disease resistance, etc., and achieve the effects of easy breeding, anesthesia suppression, and strong disease resistance

Inactive Publication Date: 2010-05-12
浙江大海洋科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the second or third generation of squid began to show the phenomenon of germplasm degradation such as individual reduction and poor disease resistance.
Especially after the third generation of artificially cultured squid, its individual individuals can only reach half of wild individuals, seriously affecting the market value

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: In this embodiment, the male golden squid is used as the male parent, and the female squid is used as the female parent. Artificial breeding method, it has the following steps:

[0020] ①Choose mature male golden squid, extract the sperm pods, and soak the sperm pods in low-temperature seawater containing adaptation reagents; the temperature of low-temperature seawater is 6°C. The adapting reagent is the sodium salt of eugenol; the concentration of the sodium salt of eugenol in the water body is 80mg / L. This operation is to infiltrate the surface of the sperm pods with the adapting agent. During the subsequent insemination operation, the adapting agent can prevent the sperm pods from falling off and improve the success rate of the insemination operation.

[0021] ②Put female squid in a container filled with low-temperature seawater for 15 minutes; the low-temperature seawater is filled with CO 2 ; Mainly to make the female squid go into dormancy, at this...

Embodiment 2

[0025] Embodiment 2: squid artificial breeding method, it has the following steps:

[0026] ①Choose mature male golden squid, extract the sperm pods, and soak the sperm pods in low-temperature seawater containing adaptation reagents; the temperature of low-temperature seawater is 7°C. The adaptive reagent is 2-phenoxyethanol; the concentration of 2-phenoxyethanol in the water body is 500mL / L, that is, 2-phenoxyethanol and water are mixed 1:1.

[0027] ②The female squid is placed in a container filled with low-temperature seawater for 10 minutes; the low-temperature seawater is filled with CO 2 ;

[0028] ③Taking out the female squid mansoni after the treatment in step ②, and implanting the sperm pods treated in step ① into the mantle of the female squid mansoni;

[0029] ④ Put the inseminated female squid back into the container of low-temperature seawater, CO 2 Inflated, and pressurized; CO 2 The inflation volume is 400mL / (min·L). The supercharging pressure is 1.1 atmosp...

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Abstract

The invention discloses a biological engineering method of squids, which comprises the steps of selecting a masculine adult squid, pinching off spermatophore thereof, and immersing the spermatophore in low-temperature seawater containing adapting reagent; putting a feminine squid in a container filled with low-temperature seawater for temporary culture for 5 to 30 minutes; charging CO2 into the low-temperature seawater; taking the feminine squid out, and implanting the spermatophore into a mantle of the feminine squid; putting the feminine squid after insemination back to the container filled with low-temperature water, charging CO2 into the seawater and boosting pressure; reducing pressure to normal, and then increasing the temperature of water gradually. The spermatophore is immersed in the low-temperature seawater containing the adapting reagent, and after insemination, failures caused by pinch-off of the spermatophore by a tentacle of a female parent due to inadaptation to the spermatophore can be effectively reduced. The biological engineering method of squids is applicable to biological engineering operation of homologous or heterogenetic squids, has the advantages of high hatching rate, high survival rate, little disease, easy culture, strong disease resistance, rapid growth and the like, and the biological engineering method has easy implementation and high success rate.

Description

technical field [0001] The invention relates to an artificial method for cephalopods, in particular to an artificial breeding method for squid. Background technique [0002] Mansoni squid was once one of the four major fisheries in my country's fishing grounds. It has strong adaptability to temperature and is widely distributed in my country's coastal areas. It is a traditional fishery species with high economic value. Due to overfishing and ecological deterioration, since the 1970s, there have been successive declines. In the 1980s, it was reported that Li Xingjie et al. conducted preliminary research on the resources and multiplication of squid squid, while there were reports on the biology and breeding of squid abroad. During the domestication and breeding of wild squid mansoni, technicians found that after two to three generations of domestication and breeding, wild squid began to adapt to the artificial domestication environment, so as to achieve the purpose of large-s...

Claims

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Application Information

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IPC IPC(8): A01K61/00
CPCY02A40/81
Inventor 吕振明董智勇吴常文迟长凤
Owner 浙江大海洋科技有限公司
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