Peste des petits ruminants virus (PPRV) reverse genetic operating system and application thereof

A technology of Peste des petits ruminants and an operating system, applied in the field of virus genetic manipulation, to achieve cost savings, significant social and economic benefits, and reduce stress responses
CN102071218AActive Publication Date: 2011-05-25HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
HARBIN VETERINARY RES INST CHINESE ACADEMY OF AGRI SCI
Publication Date
2011-05-25

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Abstract

The invention relates to a peste des petits ruminants virus (PPRV) reverse genetic operating system and an application thereof. The PPRV reverse genetic operating system comprises a transcription plasmid and one or more helper plasmids, wherein the transcription plasmid can express the genome full-length cDNA (complementary deoxyribonucleic acid) sequence of the PPRV; and the helper plasmid(s) can express nucleoprotein (N), phosphoprotein (P) and polymerase large protein (L) of the PPRV, and virus replication-permitting host cells of the PPRV. By using the PPRV reverse genetic operating system, the recombined PPRV is successfully saved. The establishment of the PPRV reverse genetic operating technical platform provides an excellent technical platform for the development of PPRV live vector vaccines and the fundamental research related to PPRV.
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Description

technical field

[0001] The invention relates to the field of virus genetic manipulation, more specifically, the invention relates to a reverse genetic operating system of Peste des petits ruminant virus and its application. Background technique

[0002] Peste des petits ruminants (PPR) is a highly contagious disease caused by Peste des petits ruminants virus (PPRV) [1]), which seriously endangers goats, sheep, and wild small ruminants. Animals such as ruminants [2] have even caused buffaloes to become ill [3].

[0003] The lethality rate of PPRV is 70-80%. In the new OIE classification, it is listed as an important economic animal disease, and the outbreak must be reported to OIE. PPR has been endangered and prevalent in Africa, the Middle East, Central Asia, South Asia and Southeast Asia for a long time. Pakistan[4] and Tajikistan[5] bordering on the west of my country have had epidemic outbreaks in recent years. In 2007, PPRV was introduced into the western border areas ...

Claims

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