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Method for preparing high content proanthocyanidin

A proanthocyanidin, high-content technology, applied in the field of chemical engineering, can solve the problems of reduced column efficiency, expensive packing, and difficulty in large-scale industrial production, and achieve the effects of low production cost, simple process flow, and easy industrialization

Inactive Publication Date: 2005-04-06
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Use dextran or C-18 bonded silica gel as a filler, although you can get higher proanthocyanidins, the price of the filler is expensive, and it is difficult to apply to large-scale industrial production; Complicated pretreatment of raw materials is required before feeding. At the same time, due to the strong adsorption of proanthocyanidins with a high degree of polymerization on polyamide, it is easy to reduce the column efficiency and require frequent regeneration with lye, which is not suitable for industrial production.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] 1) Weigh 250g of grape seeds produced in Xinjiang, add 600ml of 60% ethanol aqueous solution, stir and extract, and the extraction temperature is controlled at 40°C. The first extraction time was 60 minutes, the second and third extraction times were 20 minutes respectively, and a total of three extractions were performed. Vacuum suction filtration after each extraction is completed, the filtrates are combined, and concentrated to obtain a concentrated proanthocyanidin solution. The extract solution is evaporated under reduced pressure to remove the solvent to obtain a solvent crude extract, the proanthocyanidin content in the extract is 57.6%, and the proanthocyanidin extraction rate is 92.7%.

[0018] 2) The size of the chromatographic column is φ12×500mm, and the interior is filled with non-polar macroporous adsorption resin. The proanthocyanidin concentrated solution is passed into the chromatography column at a flow rate of 0.5 times the bed volume / hour, and the t...

Embodiment 2

[0021] 1) same as embodiment 1 step 1);

[0022] 2) The size of the chromatographic column is φ12×500mm, and the interior is filled with weakly polar macroporous adsorption resin. The proanthocyanidin concentrated solution is passed into the chromatography column at a flow rate of 0.5 times the bed volume / hour, and the temperature is controlled at 30°C. After the feeding is completed, wash the chromatography column with deionized water at a flow rate of 4.0 times the bed volume / hour; then use 40% acetone aqueous solution and pure acetone to wash the chromatography column successively at a flow rate of 4.0 times the bed volume / hour . The fraction eluted with 40% acetone was collected, concentrated and dried to obtain a product with a proanthocyanidin content of 80.6%, and a proanthocyanidin yield of 81.3%.

[0023] 3) same as embodiment 1 step 3);

Embodiment 3

[0025] 1) same as embodiment 1 step 1);

[0026] 2) The size of the chromatographic column is φ50×500mm, and the interior is filled with polar macroporous adsorption resin. The proanthocyanidin concentrated solution is passed into the chromatography column at a flow rate of 0.5 times the bed volume / hour, and the temperature is controlled at 25°C. After the feeding is completed, wash the chromatography column with deionized water at a flow rate of 1.0 times the bed volume / hour; then wash the chromatography column with 20%, 40% methanol aqueous solution and pure methanol in sequence at a flow rate of 1.0 times the bed volume / Hour. Collect all of the 20% methanol elution and a part of 40% methanol elution, concentrate and dry to obtain a product with a proanthocyanidin content of 86.2%, and a proanthocyanidin yield of 82.0%.

[0027] 3) same as embodiment 1 step 3);

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Abstract

This invention relates to a preparation method of high assay proto cyaniding. High assay proto cyaniding is got after solvent extraction, column chromatography separation and solvent precipitation process and original material is grape seed. Proto cyaniding content of solvent crude extract is about 50%, then it can be raised above 80% through macro pore polymeric adsorbent column chromatography refinery, and it can be raised above 90% through once solvent precipitation finally. proto cyaniding yield is above 75% of the whole process. The advantage is that technical process is simple, production cost is low, and it is convenient to industrialization.

Description

Technical field [0001] The invention relates to a method for preparing high-content proanthocyanidins, in particular to a method for obtaining high-content proanthocyanidins by using grape seeds as a starting material through solvent extraction, column chromatography separation and solvent precipitation, which belongs to the technical field of chemical engineering. Background technique [0002] Proanthocyanidins are polyphenolic compounds widely present in plants, and are flavan-3-ol polymers composed of catechin and epicatechin. Proanthocyanidins have strong antioxidant properties, free radical scavenging ability and ability to improve human microcirculation. It is one of the strongest and most effective oxygen free radical scavengers and lipid peroxidation inhibitors found so far. The antioxidant effect in the body is much higher than that of vitamin E and vitamin C, about 50 times that of vitamin E and 20 times that of vitamin C in the body. At present, it is widely used...

Claims

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Application Information

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IPC IPC(8): C07D311/62
Inventor 苏宝根任其龙何钊杨亦文苏云吴彩娟
Owner ZHEJIANG UNIV
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