The invention discloses a method for preserving and taking potato 
germplasm resources. The method comprises the following steps that S1, single sections of potato 
tissue culture seedlings are shearedand 
cut on a culture medium in a culture 
bottle, and a 
bottle opening is sealed by using a sealing film; S2, subculture is performed on the potato 
tissue culture seedlings treated in step S1 at the temperature of 20-22 DEG C, the illumination intensity of 3,200-3,500 lx and the illumination time of 15-18 hours per day; after two months, the illumination intensity is reduced to 2,300-2,500 lx, andculture is continued; after two months, the illumination intensity is increased to 2,700-3,000 lx; and after two months, microtuber is naturally fruited, the illumination intensity is reduced to 0, and continuous culturing is carried out until the microtuber passes away 
dormancy and starts to germinate; and S3, the microtuber starting to germinate obtained in step S2 is inoculated to a culture medium in a culture 
bottle, a bottle opening is sealed by using a sealing film, and a new round of 
germplasm resource preservation is carried out according to the operation in step S2 when the microtubergrows into a new test-tube 
plantlet. The method for preserving and taking potato 
germplasm resources is simple, convenient, easy to operate, low in cost and good in effect.